权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the Requirement and Nutritional Role of Marine Fish Larvae for EPA and DHA

海水鱼幼体对EPA和DHA的需要量及营养作用的研究

基本信息

批准号：
02660208
负责人：
TAKEUCHI Toshio
金额：
$ 1.41万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660208/
关键词：
EPA DHA Requirement Nutrition Marine Fish Larvae Artemia マダイシマアジワムシ

项目摘要

This study was investigated the requirement and nutritional role of marine fish larvae for icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).At first, different stages of larval fish such as striped jack, yellowtail, cod were collected and analysed their fatty acid compositions. Only DHA content in three species was decreased immediately after hatching. This result suggests that fish larvae during 5-10mm size (total length) may require high amount of DHA.Secondly, the experiment was conducted to find out a suitable lipid type for enrichment by comparing the incorporation of lipid and n-3highly unsaturated fatty acids (n-3HUFA) in Artemia nauplii between various types of oil, such as ethylesters (EE), triglycerides (TG), methyl esters (ME), and free fatty acids (FFA) containing about 43% of n-3HUFA, using the so-called direct method. It is concluded that a maximum incorporation of n-3HUFA in Artemia can be obtained by enrichment with EE for 24h and that EE or ME are very quickly … More incorporated into TG fractions. Then these Artemia were fed on larval striped knife jaw and red sea bream to compare the dietary value of Artemia enriched with various types of oil, based on the growth, survival and chemical compositions of both fish larvae. The result indicated that the dietary value of Artemia to both species was effectively improved by enrichmewith TG, ME, and EE, especially with EE.In next, dietary value of Artemia enriched with EE type of EPA and DHA for larval yellowtail and red sea bream was investigated. Both larval yellowtail and red sea bream fed on nauplii enriched with EPA showed good growth and high survival rate, however quite low activity tests were observed. On the other hand, fish fed DHA enriched Artemia showed high feed performances, incl. high activity tests. Thus, it is clearly indicated that larval fish require DHA in order to receive good growth and health.Finally, two feeding experiments were conducted to compare the essential fatty acids (EFA) value between EPA and DHA in juvenile red sea bream and striped jack. Both fish fed a diet without supplemental EFA showed low feed efficiency and high morthlity. The requirement of EPA and DHA was estimated to be around 1% and 0.5% in diet for juvenile red sea bream, respectively. The EFA efficiency of DHA was also found to be twice as high as that of EPA. On the other hand, the experiment on the EFA of juvenile striped jack have demonstrated that : 1) DHA is more effective than EPA as EFA ; 2) requirement for DHA alone is about 1.7% in diet.This study was clearly demonstrated that marine fish larvae require DHA as EFA and physiological purpose. Less

为了研究海水鱼仔稚鱼对二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的需要量及其营养作用，采集了不同发育阶段的条纹鱼、黄尾鱼、鳕鱼等仔稚鱼，分析了其脂肪酸组成。只有DHA含量在孵化后立即下降。这一结果表明，在5- 10毫米大小的鱼苗其次，通过比较不同类型的油（例如，乙酯（EE）、甘油三酯（TG）、甲酯（ME））之间的脂肪和卤虫无节幼体中的n-3高度不饱和脂肪酸（n-3 HUFA）的掺入，进行实验以找出适合富集的脂质类型，和含有约43%的n-3 HUFA的游离脂肪酸（FFA）。结果表明，用EE富集24 h，卤虫体内n-3 HUFA的掺入量最大，EE或ME对卤虫体内n-3 HUFA的掺入量都很迅速。 ...更多信息掺入TG馏分中。然后将这些卤虫分别饲喂条纹刀颌鱼和真鲷幼鱼，根据两种鱼幼鱼的生长、存活和化学成分，比较富含不同类型油脂的卤虫的膳食价值。结果表明，添加TG、ME和EE均能有效提高卤虫对黄尾鱼和真鲷的饵料价值，其中EE的效果最好。黄尾和真鲷幼鱼在添加EPA的无节幼体上生长良好，成活率高，但活性试验结果很低。另一方面，喂食富含DHA的卤虫的鱼显示出高饲料性能，包括：高活性测试因此，幼鱼需要DHA才能获得良好的生长和健康。最后，通过两个饲养试验比较了真鲷幼鱼和条纹鱼幼鱼EPA和DHA的必需脂肪酸（EFA）值。这两种鱼饲料中没有补充EFA表现出低饲料效率和高死亡率。真鲷幼鱼对EPA和DHA的需要量分别为1%和0.5%左右。DHA的EFA效率也是EPA的两倍。另一方面，对幼鱼必需脂肪酸的研究表明：1）DHA作为必需脂肪酸比EPA更有效; 2）DHA单独需要量约为饲料的1.7%，这一研究清楚地证明了海水鱼幼鱼需要DHA作为必需脂肪酸和生理目的。少