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Molecular biology mark study on development of digestive and absorptive function of mothr's milk fed rat pups intestine : Developmental changes in protein -Effect of neonatal undernutrition on the subsquent development of protein digestive and absorptive

母乳喂养幼鼠肠道消化吸收功能发育的分子生物学标志研究：蛋白质发育变化-新生儿营养不良对蛋白质消化吸收功能后续发育的影响

基本信息

批准号：
05670975
负责人：
TAKEUCHI Toshio
金额：
$ 1.47万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

We have examined the effect of neonatal undernutrition on protein degestive and absorptive function of rat intestine. Eight pups were reared at a litter in the control group, while 16 pups in the undernourished group were reared for 1 week commencing at the age of 2, and then 8 pups were reared as a litter. Protein digestive function of intestinal lumen (proteolytic activity), the protein absorptive function (pinocytic activity) by using everted small intestinal sac, and the Cathepsin B activity of intestinal mucosa and liver were measured at 2,9,16 and 23 days in control and undernourished pups.The proteolytic activity was 7.6,177.3,530.6 (mu g/tripsin equivalent/ml) in the control group, respectively. However, the value of undernourished group showed decreases compared with those in the control group (70.3,46.8 and 98.5% of each opposite control). The mucosal uptake amount of RI labeled BSA at 9 and 16 days were increased significantly in the undernourished group (5.6,1.9 times of control), but the amount of that at 23 days was not significantly defferent from control. On the other hand, that of RI labeled BLG was more increased compared with each opposite control (69.6,2.3,3.4 times). The Cathepsin B activity (U/mg protein/ml) of the control liver at 9,16,23 days showed 10.4,18.1,9.7. The activity of the undernourished group was significantly declined on these days (7.9,10.0,7.1). While, that of intestinal mucosa groups showed significant decreases compared with those in control group at 9 and 16 days (4.4,4.3), but significant increases (2 times the control value) at 23 days.These studies suggest that neonatal undernutrition may have unfavorable influences on the subsequent development of protein digestive and absorptive function, and may be a factor in development of future allergic disease.

本文研究了新生期营养不良对大鼠小肠蛋白质消化吸收功能的影响。对照组每窝饲养8只幼仔，而营养不良组的16只幼仔从2岁开始饲养1周，然后每窝饲养8只幼仔。肠腔蛋白质消化功能（蛋白水解活性），蛋白质吸收功能在对照组和营养不良组的第2、9、16和23天，分别测定了小肠粘膜和肝脏组织蛋白酶B活性，蛋白水解活性分别为7.6、177.3、530.6（μ g/胰蛋白酶当量/ml）。而营养不良组则明显低于对照组，分别为对照组的70.3%、46.8%和98.5%。营养不良组大鼠胃粘膜对RI标记BSA的摄取量在第9、16天显著增加（分别为对照组的5.6、1.9倍），但在第23天与对照组无显著差异。而RI标记的BLG细胞则比各对侧对照组细胞的荧光强度增加了69.6，2.3，3.4倍。对照组肝组织蛋白酶B活性（U/mg蛋白/ml）在9、16、23 d分别为10.4、18.1、9.7。营养不良组的活动在这些天显著下降（7.9，10.0，7.1）。而肠粘膜组在第9、16天较对照组显著降低（4.4，4.3），第23天较对照组显著升高（2倍于对照值），提示新生儿营养不良可能对以后蛋白质消化吸收功能的发育产生不利影响，并可能是日后发生变态反应性疾病的一个因素。