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Studies on the environmental control for the mass propagation of the tissue cultured plantlets by direct ex-vitro rooting method

直接离体生根法组培苗大量繁殖的环境控制研究

基本信息

批准号：
02660259
负责人：
HAYASHI Makio
金额：
$ 1.28万
依托单位：
Tokai University (1991)Chiba University (1990)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

In this study, the mass propagation of the tissue cultured plantlets by direct ex-vitro rooting method were discussed. In this method, the explants, single node cuttings each with leaves, excised from the plantlets in vitro were inserted in rockwool plugs in the plug tray and were transferred directly to the environmental control unit and were cultured with a (sugar free) diluted nutrient solution for hydroponics.On the first year, the effects of solar radiation and CO_2 concentration on the growth of potato plantlets with the ex-vitro direct rooting method was examined. Explants were cultured inside the environmental control unit at different intensities of solar radiation and CO_2 concentrations. All the explants grew to the plantlets and survived in each treatment. The growth of the plantlets with this method were compared with the growth of the plantlets with the conventional method. The plantlets grew faster and were more vigorous under high solar radiation and CO_2 enriched conditions in direct exvitro rooting method than that under general conditions in conventional method. It was shown that the plantlets have a photosynthetic ability and they can grow autotrophically on the sugarfree medium if the physical environment is properly controlled for the autotrophic growth by photosynthesis.On the second year, the effects of the volume and surface area of the artificial substrate, which affects the content of nutrient and dissolved oxygen in the substrate, on the growth of plantlets was also examined. It was shown that the volume and surface area of the substrate affected the growth of the plantlets. It seems that the further investigation about the effects of the root zone environment on the growth of the plantlets. It seems that the investigation about woody plants as explants will be needed.

本文对试管苗直接生根法组培快繁技术进行了探讨。该方法从马铃薯试管苗中取出外植体，每条带叶的单节插条插入插盘的岩棉塞中，直接转移到环境控制单元，然后用(无糖)稀释营养液进行水培培养。在不同的太阳辐射强度和CO_2浓度下，在环境控制单元内培养外植体。所有的外植体都长到了植株上，并在各个处理中存活下来。并与常规方法培养的试管苗生长情况进行了比较。在高太阳辐射和高CO_2浓度条件下，直接生根法比常规生根法生长更快，活力更强。结果表明，通过光合作用控制适宜的物理环境有利于自养生长，试管苗具有一定的光合作用能力，并能在无糖条件下自养生长。第二年，考察了人工基质的体积和表面积对试管苗生长的影响，并考察了基质中养分含量和溶解氧的影响。结果表明，基质的体积和表面积对试管苗的生长有影响。因此，根区环境对试管苗生长的影响有待进一步研究。因此，木本植物作为外植体的研究势在必行。