Studies on the environmental control for the mass propagation of the tissue cultured plantlets by direct ex-vitro rooting method

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In this study, the mass propagation of the tissue cultured plantlets by direct ex-vitro rooting method were discussed. In this method, the explants, single node cuttings each with leaves, excised from the plantlets in vitro were inserted in rockwool plugs in the plug tray and were transferred directly to the environmental control unit and were cultured with a (sugar free) diluted nutrient solution for hydroponics.On the first year, the effects of solar radiation and CO_2 concentration on the growth of potato plantlets with the ex-vitro direct rooting method was examined. Explants were cultured inside the environmental control unit at different intensities of solar radiation and CO_2 concentrations. All the explants grew to the plantlets and survived in each treatment. The growth of the plantlets with this method were compared with the growth of the plantlets with the conventional method. The plantlets grew faster and were more vigorous under high solar radiation and CO_2 enriched conditions in direct exvitro rooting method than that under general conditions in conventional method. It was shown that the plantlets have a photosynthetic ability and they can grow autotrophically on the sugarfree medium if the physical environment is properly controlled for the autotrophic growth by photosynthesis.On the second year, the effects of the volume and surface area of the artificial substrate, which affects the content of nutrient and dissolved oxygen in the substrate, on the growth of plantlets was also examined. It was shown that the volume and surface area of the substrate affected the growth of the plantlets. It seems that the further investigation about the effects of the root zone environment on the growth of the plantlets. It seems that the investigation about woody plants as explants will be needed.