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Studies on the environmental control for the mass propagation of the tissue cultured transplants by direct ex-virto rooting method

组培直接离体生根法大量繁殖的环境控制研究

基本信息

批准号：
07660349
负责人：
HAYASHI Makio
金额：
$ 1.47万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

In this study, the environmental control for the mass propagation of the tissue culture plantlets by 'direct ex-vitro rooting method' was discussed. In the direct ex-vitro rooting method, the shoot elongation, rooting, and acclimatization of the explants/plantlets are simultaneously achieved in the environmental control unit under the photoautotrophic condition. The following results were taken from the experiments.1) The effects of the photosynthetic photon flux density (PPFD) and concentration oi culture solution on the growth of strawberry plantlets were examined. The explants of micropropagated shoots were directly inserted into rockwool plugs and cultured inside the environmental control unit by hydroponics using a culture solution at different concentrations. The effect of the concentration of culture solution on the growth of plantlets was obvious in high PPFD than that in low PPFD.2) The transpiration rate of the explants/plantlets was changed by wind speed at a certain high relative humidity. In order to reduce the death rate and to increase the growth rate of the plantlets, the control of the wind speed near plantlets seems to be as important as the control of humidity.3) The conditions to repress the death of plantlets were discussed and the various informations were obtained.4) The relationship between the weight of explants and the growth after planting was examined in this method. The relative growth rate (RGR) was promoted in the treatment of relatively low weight of the explants. It seems that the culture period will be shortened and small transplant will be produced in this method than in the conventional method.

本研究探讨了“直接离体生根法”组培苗大量繁殖的环境控制。在直接离体生根方法中，在光自养条件下的环境控制单元中同时实现外植体/小植株的芽伸长、生根和驯化。主要研究结果如下：1）研究了光合光子通量密度（PPFD）和培养液浓度对草莓试管苗生长的影响。将微繁殖芽的外植体直接插入岩棉塞中，并在环境控制单元内使用不同浓度的培养液通过水培进行培养。培养液浓度对组培苗生长的影响在高PPFD条件下比低PPFD条件下明显。2）在一定的高相对湿度条件下，风速对外植体/组培苗的蒸腾速率有一定的影响。为了降低组培苗的死亡率，提高组培苗的生长速度，控制组培苗附近的风速与控制湿度同样重要。3）探讨了抑制组培苗死亡的条件，获得了各种信息。相对生长速率（RGR）在相对低的外植体重量的处理中被促进。与常规方法相比，该方法可缩短培养周期，并产生较小的移植物。