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Studies on the environmental control for acclimatization of the tissue culture plantlets.

组培苗驯化的环境控制研究。

基本信息

批准号：
62560252
负责人：
HAYASHI Makio
金额：
$ 1.47万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560252/
关键词：
Tissue culture Environmental control Acclimatization 湿度環境順化装置組織培養炭酸ガス

项目摘要

1. The acclimatization unit was developed to give better environmental control, higher survival rates and more rapid growth of plantlets(Kozai et al, 1987).Using the acclimatization unit, the test cultivation were carried out to investigate the effect of CO_2 enrichment during the acclimatization period on the growth of the propagated plantlets. The plantlets (chrysanthemum and ginger) were acclimatized in the acclimatization unit with and without CO_2 enrichment. The dry weight, number of leaves, and plant height of plantlets with the CO_2 enrichment were greater than those without enrichment. It is considered that the acclimatization period can be shortened by CO_2 enrichment because the CO_2 enrichment promotes growth of the plantlets.2. Explants, single node cuttings each with two leaves, of carnation excised from the plantlets in vitro were inserted in rockwool plugs and were transferred directly to the acclimatization unit and were cultured with a (sugar free) diluted nutrient solution for hydroponics. The growth of the plantlets with this method was compared with the growth of the plantlets with the conventional method. The conventional method here means that the explants are cultured and grown to the plantlets on the medium with sugar in vitro then acclimatized ex vitro with the (sugar free) diluted nutrient solution.The plantlets grew faster and were more vigorous with the acclimatization unit method than those with the conventional method. Then the method proposed in this study for preparation (differentiation, growth and rooting) and acclimatization of the carnation plantlets can be substituted for the conventional method in many cases and may be applied for other plant species.

1. 驯化装置的开发是为了更好的环境控制，更高的成活率和更快的幼苗生长（Kozai et al, 1987）。利用驯化装置进行了盆栽试验，研究了驯化期CO_2富集对繁殖苗生长的影响。在有CO_2富集和没有CO_2富集的驯化单元中对菊花和生姜进行了驯化。CO_2富集处理的植株干重、叶片数和株高均大于未富集处理的植株。认为CO_2富集可以缩短驯化期，因为CO_2的富集促进了植株的生长。从离体植株上切除的康乃馨的外植体，每根有两片叶子的单节插条，插入岩棉塞中，直接转移到驯化单元，用（无糖）稀释的营养液进行水培培养。用该方法与常规方法进行了苗的生长情况比较。这里的常规方法是将外植体培养到离体培养基上，然后用（无糖）稀释的营养液进行离体驯化。驯化单元法比常规驯化单元法植株生长更快、更健壮。因此，本研究提出的康乃馨苗的制备（分化、生长、生根）和驯化方法在许多情况下可替代传统方法，并可应用于其他植物品种。