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Biliary Tract Disease and Compound Carbohydrates - Immunohistochemical and Collagen Gel Culture Studies -

胆道疾病和复合碳水化合物 - 免疫组织化学和胶原蛋白凝胶培养研究 -

基本信息

批准号：
02670135
负责人：
NAKANUMA Yasuni
金额：
$ 1.6万
依托单位：
KANAZAWA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The present project was aimed to correlate the biliary tract diseases and biliary epithelial expression of carbohydrate antigens using immunohistochemstry and collagen gel culture methods. The following results were obtained. 1. A, B and H blood group related carbohydrate antigens were frequently found in the intrahepatic large and septal bile ducts but rare in the small bile ducts of normal livers. However, such expressions were frequent and intense in the diseased intrahepatic small bile ducts irrespective their etiologies. Lewis a and b containing type I chain were expressed diffusely along the normal and abnormal intrahepatic bile ducts. Lewis X and Y containing type II chain were rarely found in the intrahepatic large bile duct in normal livers, though Lewis Y became strongly expressed on the pathologic bile ducts irrespective of their sizes or etiologies. 2. Cancer related carbohydrate residues such as CEA, CA 19-9 and DUPAN II were frequently seen in cholagniocarcinoma associate … More d with hepatolithiasis, and also their preneoplastic lesions in stone-containing intrahepatic bile ducts. ABH, and Lewis a, b, X, and Y blood group related antigens were lost or strongly expressed clonally in carcinoma tissue, while such clonal expression was not evident in atypical biliary epithelial hyperplasia of the stone-containing bile ducts. 3. Biliary epithelial cells isolated from rabbits and cultured in collagen gel lost transiently their polarity, while they profiliferated and formed unicellular and multicellular cysts, and then they regained their pecular polarities manifested by the expression of carbohydrate residues, microvilli and other markers of cellular polarity. Preliminary study disclosed that carcer cell lines (CCKS 1) from the biliary tract cancer did not show the peculiar carbohydrate distribution and secretion as seen in rabbit biliary epithelial cells after embedding and culture in collagen gel culture. 4. Subculture of individual anatomial elements of biliary epithelial cells from the biliary tract diseases in the colagen gel culture and the altered secretion and production of carbohydrate secretion under various stimuli seems necessary to evaluate the pathophysiology of the intrahepatic biliary tree. Less

本研究旨在通过组织化学和胶原凝胶培养方法，探讨胆道疾病与胆道上皮糖抗原表达的关系。获得了以下结果。1. A、B和H血型相关的糖类抗原在正常肝内大胆管和隔胆管中多见，而在小胆管中少见。然而，这种表达是频繁和强烈的病变肝内小胆管无论其病因。含I型链的刘易斯a和B在正常和异常肝内胆管呈沿着弥漫性表达。在正常肝脏肝内大胆管中很少发现含有刘易斯X和Y的II型链，尽管刘易斯Y在病理性胆管上变得强烈表达，而与它们的大小或病因无关。2.癌相关糖残基如CEA、CA 19-9和DUPAN Ⅱ在胆管癌相关性中常见 ...更多信息 d肝内胆管结石，以及含石肝内胆管癌前病变。ABH和刘易斯a、B、X和Y血型相关抗原在癌组织中缺失或克隆性强表达，而在含石胆管的不典型胆管上皮增生中这种克隆性表达不明显。3.从兔体内分离的胆管上皮细胞在胶原凝胶中培养时，其极性短暂丧失，但细胞增殖并形成单细胞和多细胞囊，然后恢复其特有的极性，表现为糖残基、微绒毛和其他细胞极性标志物的表达。胆管癌细胞系CCKS-1经胶原凝胶包埋培养后，未显示出兔胆管上皮细胞特有的糖分布和分泌。4.在胶原凝胶培养中对来自胆道疾病的胆管上皮细胞的个体解剖学成分进行继代培养，以及在各种刺激下改变碳水化合物分泌的分泌和产生，似乎对评价肝内胆管树的病理生理学是必要的。少