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Functional and morphological changes of arterial endothelial cells in chronic hypertension.

慢性高血压中动脉内皮细胞的功能和形态变化。

基本信息

批准号：
02670149
负责人：
HAZAMA Fumitada
金额：
$ 1.6万
依托单位：
Shiga University of Medical Science, School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

(1)Endothelial cells of both the aorta and cerebral microvessels in spontaneously hypertensive rats (SHR) proved to have increased turn-over rateds compared with those in the controls.(2)The activity of endothelial cell-derived growth factors (ECDGFs) in cultured aortic endothelial cells fro SHR was studied by bioassaying the proliferative effect of endothelial cellconditioned medium on vascular smooth muscle cells, fibroblasts and astrocytes. Such cells treated with the conditioned medium showed an accelerated proliferation compared with controls.(3)PDGF-B chainrelated growth factor proved to constitute a major portion of the mitogenic activity of the conditioned media of endothelial cells from both SHR and the control rats.(4)Nothern analysis revealed that the expression of PDGF-B chain was markedly enhanced in cultured endothelial cells of SHR.(5)The fact that the release of ECDGFs from cultured aortic endothelial cells was markedly increased by treatment with a tumor-promoting phorbol ester indicates the participation of protein kinase C in the regulation of release of ECDGFs from the endothelial cells. Based on our above-mentioned findings, we may suppose that the increased growth factors, such as PDGF.B chanin and others, are produced and released in chronic hypertension and they play an important role in the pathogeneses of atherosclerosis and hypertensive arteriolosclerosis.

（1）自发性高血压大鼠（SHR）主动脉和脑微血管内皮细胞的转换率均高于对照组。（2）通过检测内皮细胞条件培养液对血管平滑肌细胞、成纤维细胞和星形胶质细胞增殖的影响，研究内皮细胞衍生生长因子（ECDGFs）在培养SHR主动脉内皮细胞中的活性。与对照相比，用条件培养基处理的这些细胞显示出加速的增殖。（3）PDGF-B链相关生长因子是SHR和对照组内皮细胞条件培养液促有丝分裂活性的主要成分。（4）Northern分析显示，培养的自发性高血压大鼠内皮细胞中PDGF-B链的表达明显增强。（5）通过用促肿瘤的佛波醇酯处理，ECDGF从培养的主动脉内皮细胞的释放显著增加，这一事实表明蛋白激酶C参与调节ECDGF从内皮细胞的释放。根据上述研究结果，我们认为，PDGF、B chanin等生长因子在慢性高血压时大量产生和释放，在动脉粥样硬化和高血压小动脉硬化的发病中起重要作用。