Participation of Endogenous Opioid Peptide in Sudden Death

内源性阿片肽参与猝死

• 批准号: 02670264
• 负责人: TSUNODA Kenji
• 金额: $ 1.15万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670264/
• 关键词: Sudden Death; beta-Endorphin; Hypothalamic-Pituitary System; Respiratory Failure; Cardiac Failure; Agonal Period; Sex Difference; Sympathoinhibitory Effect; βーエンドルフィン; 呼吸抑制; 循環障害; 脳室内投与; ナロキソン; ラット; オピオイドペプチド; βーendorphin; 心臓死; 呼吸死; 間脳下垂

Using male and female Wistar rats, pituitary response to cardiac and respiratory failure type (CFT and RFT) sudden death caused by the intravenous administration of KCl and SCC, respectively, was examined by analyzing variation in pituitary immunoreactive beta-endorphin (IR-beta-EP) levels determined by radioimmunoassay after death and in circulating IR-beta-EP levels during periods of agony. In the pituitary gland of both sexes which differed significantly in ratio of the organ weight to body weight for CFT and RFT (CFT>RFT), IR-beta-EP was significantly less in RFT than in CFT (P 0.05). No variation in plasma IR-beta-EP was noted during short periods of agony in CFT, but it markedly increased during long periods of agony in RFT. The highest elevation at 2 or 4 minutes after SCC administration was about 3 times the preadministration value for IR-beta-EP in males (P 0.01). But elevation in females was lower than in males. Rise in plasma IR-beta-EP during agony of RFT is regarded to be … More of pituitaryorigin due to dexamethasone treatment. The pituitary was thus concluded to respond more to the fatal agony in RFT than in CFT.An attempt was made to determine sex differences in the effects of beta-endorphin ( beta-EP) on respiratory and cardiovascular systems using the same strain rats. By the intracerebroventicular administration of beta-EP (0.18 mg/kg) to normal male and female rats, respiratory rate (RR), heart rate (HR) and mean arterial blood pressure (MABP) were depressed significantly (P 0.01 or 0.001) and CO_2 in expired gas increased significantly (P 0.01). These suppressive effects of the peptide were transiently blocked by the intravenous injection of naloxone (0.2 mg/kg). No differences in the effects of beta-EP between estrous and diestrous female rats could be detected. The effects of the peptide were significantly stronger in RR, HR and MABP for females than for males (0.001 P 0.05). Testectomized rats showed suppressive effects of the peptide to the same extent as intact females, but the effects in overiectomized rats did not differ from those for intact females. In testectomized and ovariectomized rats treated with testosterone, the former showed the same results as intact males, but not the latter. The suppressive effects of beta-EP on the respiratory and cardiovascular systems are thus remarkably relieved by androgen in male rats. Less

采用雄性和雌性Wistar大鼠，通过分析死亡后放射免疫测定的垂体免疫反应性β-内啡肽（IR-β-EP）水平和痛苦期间循环IR-β-EP水平的变化，研究了垂体对分别由KCl和SCC静脉给药引起的心脏和呼吸衰竭型（CFT和RFT）猝死的反应。在CFT和RFT（CFT>RFT）的器官重量/体重比值显著不同的两种性别的垂体中，RFT中的IR-β-EP显著低于CFT（P 0.05）。在CFT的短时间痛苦期间，血浆IR-β-EP没有变化，但在RFT的长时间痛苦期间，血浆IR-β-EP显著增加。SCC给药后2或4分钟的最高升高约为雄性IR-β-EP给药前值的3倍（P 0.01）。但女性的升高低于男性。RFT疼痛期间血浆IR-β-EP升高被认为是 ...更多信息 垂体源性的，由于地塞米松治疗。本实验用同种大鼠研究了β-内啡肽（β-EP）对呼吸和心血管系统的作用的性别差异。侧脑室注射β-EP（0.18mg/kg）可使正常大鼠呼吸频率（RR）、心率（HR）和平均动脉压（MABP）显著降低（P0.01或0.001），呼出气中CO_2浓度显著升高（P0.01）。静脉注射纳洛酮（0.2 mg/kg）可短暂阻断肽的这些抑制作用。在动情期和非动情期雌性大鼠之间未检测到β-EP的作用差异。女性的RR、HR和MABP的作用显著强于男性（0.001 P 0.05）。睾丸切除大鼠显示出与完整雌性相同程度的肽抑制作用，但在过度切除大鼠中的作用与完整雌性没有差异。在睾丸切除和卵巢切除大鼠睾酮治疗，前者表现出相同的结果，作为完整的男性，但不是后者。因此，雄激素可显著缓解β-EP对雄性大鼠呼吸和心血管系统的抑制作用。少

项目成果

Kenji Tsunoda: "beta-Endorphin Secretion at the Time of Sudden Death due to Cardiac or Respiratory Failure." Jpn J Legal Med. 46. 182-188 (1992)

Kenji Tsunoda：“由于心脏或呼吸衰竭而猝死时β-内啡肽的分泌。”

Kenji Tsunoda: "Sex Differences in Respiratory and Cardiovascular Effects of beta-Endorphin." Jpn J Legal Med. 47. (1993)

Kenji Tsunoda：“β-内啡肽对呼吸和心血管影响的性别差异。”

Kenji TSUNODA: "β-Endorphin Secretion at the Time of Sudden Death due to Cardiac or Respiratory Failure." Jpn J Legal Med. 46. 182-188 (1992)

Kenji Tsunoda：“由于心脏或呼吸衰竭而猝死时的β-内啡肽分泌。” Jpn J Legal Med 46. 182-188 (1992)

Kenji TSUNODA: "Sex Differences in Respiratory and Cardiovascular Effects of β-Endorphin." Jpn J Legal Med. 47. (1993)

Kenji Tsunoda：“β-内啡肽对呼吸和心血管影响的性别差异。”Jpn J Legal Med 47。（1993）

角田 健司,黒岩 康子,道解 公一,李 暁鵬,秋谷 行男,渡辺 富雄: "ラット脳室内βーendophin投与による呼吸・循環動態の変化ーその性差についてー" 日本法医学雑誌. 46. (1992)

Kenji Tsunoda、Yasuko Kuroiwa、Koichi Michikae、Xiaopeng Li、Yukio Akiya、Tomio Watanabe：“脑室内β-内啡肽给药引起的大鼠呼吸和血流动力学变化 - 关于其性别差异”《日本法医学杂志》46。（1992 年） ）