Regulation Mechanism on Growth and Differentiation of Pancreatic Cancer Cells

胰腺癌细胞生长分化的调控机制

• 批准号: 02670300
• 负责人: SAITO Seiji
• 金额: $ 1.41万
• 依托单位: Toyama Medical and Pharmaceutical University, University Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670300/
• 关键词: Pancreatic cancer; Cell-kinetics; Cell-biology; Differentiation-induction; Cultured cell line; Glycoconjugates; Intracellular communication; Extracellular matrix; 細胞増殖動態; 基底膜物質; BraU; DNA合成; レクチン

The results of the study using experimental pancreatic cancer models, hamster and human cell lines established by the authors, were summarized as follows;1) Basement membrane matrix (MATRIGEL^<TM>) induced the differentiation of pancreatic cancer cell lines, both of hamster and human origin, by morphological study and cell-kinetic analysis using BrdU cumulative labeling method.2) The influences of various gut peptides, steroid hormones, and growth factors on growth and morphology of pancreatic cancer cells depended on each cell line.3) The differentiated cancer cells induced by basement membrane matrix showed increased number of gap junctions by electron-microscopy.4) Results of quantification of intracellular Ca++ and cyclic AMP were indefinite.5) In vivo growth characteristics of the differentiated hamster pancreatic cancer cells by basement membrane matrix, showed no significant difference compared with control cells, when injected into syngeneic animals. This result indicates that differentiation-induction by basement membrane matrix is reversible.These results should contribute to the further progress in the research of pancreatic cancer cell biology.

用作者建立的实验性胰腺癌模型、仓鼠和人细胞系进行的研究结果总结如下：1)基底膜基质(MATRIGEL^&lt；TM&GT；2)不同肠肽、类固醇激素和生长因子对胰腺癌细胞生长和形态的影响因细胞系不同而异。3)基底膜基质诱导分化的癌细胞在电子显微镜下可见细胞间隙连接增多。4)细胞内钙离子和环磷酸腺苷的定量结果不确定。5)基底膜基质诱导分化的仓鼠胰腺癌细胞体内生长特性与对照细胞相比，差异无统计学意义。这一结果表明，基底膜基质诱导分化是可逆的，这一结果将有助于胰腺癌细胞生物学研究的进一步发展。

项目成果

Yamazaki.K,Saito S,et,al.: "Cell kinetic analysis of pancreatic cancers in Syrian golden hamsters" International Journal of Pancreatology.

Yamazaki.K，Saito S，et，al.：“叙利亚金仓鼠胰腺癌的细胞动力学分析”国际胰腺学杂志。

Kunio YAMAZAKI: "Cell-kinetic analysis of Pancreatic cancers in syrian golden hamsters." Int.J.Pancreatol.10. 121-132 (1991)

Kunio YAMAZAKI：“叙利亚金仓鼠胰腺癌的细胞动力学分析。”

Nobuyuki NISHIMURA: "Newly established human pancreatic carcinoma cell lines and their lectin binding properties." Int.J.Pancreatol.(1993)

Nobuyuki NISHIMURA：“新建立的人类胰腺癌细胞系及其凝集素结合特性。”

田口 恭仁子: "ハムスター膵導管腺癌由来培養細胞の分化増殖に及ぼす基底膜物質の影響." 膵臓.(1993)

Kyoko Taguchi：“基底膜物质对仓鼠胰腺导管腺癌培养细胞分化和生长的影响”（1993）。

Kunio Yamazaki,: "CellーKinetic analysis of Pancreatic cancers in syrian golden hamsters." Int.J.Pancreatol.10. 121-132 (1991)

Kunio Yamazaki，：“叙利亚金仓鼠胰腺癌的细胞动力学分析。”Int.J.Pancreatol.10（1991）。