Basic and Clinical Research for Peripheral Blood Stem Cell Transplantation

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We performed basic and clinical research of peripheral blood stem cell transplantation (PBSCT) as treatment in childhood malignant disorders. For clinical research, we performed PBSCT in six cases with refractory acute leukemia and four cases of solid tumors. Four cases with acute leukemia and 2 cases with solid tumors have disease-free survival. We consider that conventional therapy is not effective for these patients and PBSCT is effective. For collection of peripheral blood stem cell efficacy will be poor after serial apheresis or recurrent chemotherapy in previous reports. But our results indicate that sufficient stem cells can be collected after recurrent chemotherapy or serial apheresis. The number of periperal blood stem cells(CFU-GM, BFU-E, CFU-Mix) increased fiftyfold to one-hundredfold using CD34 antibody. However total number of stem cells reduced after enrichment. Possibility of association between peripheral CD34 positive cells and the number of CFU-GM was suggested. The secondary granulocyte-macrophage colonies were formed using cells derived from single CFU-MIX.