Control of hematopoietic progenitors and molecular mechanism Influencing cell cycle

本研究的目的是确定血小板生成素 (Tpo) 与其他细胞因子联合使用对小鼠巨核细胞、粒细胞-巨噬细胞、红细胞和原始造血祖细胞生长的影响，排除血清因子协同作用的可能性。无血清培养使重组小鼠 (rm) Tpo 能够实现巨核细胞集落生长，这得到了与rmIL-3、rmSCF 或 rhEpo。重组人 IL-6、rhIL-11 和 rmGM-CSF 仅在血清存在下才与 rmTPO 表现出协同作用。 RmIL-3和rm SCF增加了含有其他群体的大集落和混合型集落，表明这些细胞因子促进了CFU-Mk未成熟群体的增殖。 rmTpo 增强了 rmGM-CSF 或 rmIL3 刺激的 GM 集落的生长，以及 rhEpo（有或没有 rmIL-3）刺激的红细胞爆发。仅在血清或 rmSCF 存在的情况下，rmTpo 还可以与 rmIL-3 协同显着增加 HPP 集落。

The aim of the present study was to determine the effect of thrombopoietin(Tpo) in combination with other cytokines on the growth of murine megakaryocytic, granulocyte-macrophage, erythroid and primitive hematopoietic progenitor cells, excluding possibilities of synergistic effects by serum factor(s).Serum-free culture enabled magakaryocyte colony growth by recombinant murine(rm) Tpo and this was synergistically supported with rmIL-3, rmSCF or rhEpo. Recombinant human IL-6, rhIL-11 and rmGM-CSF showed synergistic effects with rmTPO only In the presence of serum. RmIL-3 and rm SCF Incresed the large colonies and mixed-type colonies containing other populations, suggesting that these cytokines promoted the proliferation of Immature populations of CFU-Mk. The rmTpo enhanced the growth of GM-colonies stimulated by rmGM-CSF or rmIL3 and erythroid bursts by rhEpo, with or without rmIL-3. The rmTpo also significantly incresed HPP colonies in synergy with rmIL-3 only in the presence of serum or rmSCF.