Control of hematopoietic progenitors and molecular mechanism Influencing cell cycle

造血祖细胞的调控及影响细胞周期的分子机制

• 批准号: 10670744
• 负责人: IKUTA Koichiro
• 金额: $ 1.86万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670744/
• 关键词: HEMATOPOIETIC PROGENITORS; HIGH PROLIFERATIVE POTENTIAL COLONY FORMING CELLS; SERUM-FREE CULTURE; thrombopoietin; 臍帯血; CD34; サイトカイン; HPP-CFC; 造血幹細胞; 増殖分化動態; 加齢 マウス; 幼若マウス; 増殖動態

The aim of the present study was to determine the effect of thrombopoietin(Tpo) in combination with other cytokines on the growth of murine megakaryocytic, granulocyte-macrophage, erythroid and primitive hematopoietic progenitor cells, excluding possibilities of synergistic effects by serum factor(s).Serum-free culture enabled magakaryocyte colony growth by recombinant murine(rm) Tpo and this was synergistically supported with rmIL-3, rmSCF or rhEpo. Recombinant human IL-6, rhIL-11 and rmGM-CSF showed synergistic effects with rmTPO only In the presence of serum. RmIL-3 and rm SCF Incresed the large colonies and mixed-type colonies containing other populations, suggesting that these cytokines promoted the proliferation of Immature populations of CFU-Mk. The rmTpo enhanced the growth of GM-colonies stimulated by rmGM-CSF or rmIL3 and erythroid bursts by rhEpo, with or without rmIL-3. The rmTpo also significantly incresed HPP colonies in synergy with rmIL-3 only in the presence of serum or rmSCF.

本研究的目的是确定血小板生成素（Tpo）与其他细胞因子联合对小鼠巨核细胞、粒细胞-巨噬细胞、红细胞和原始造血祖细胞生长的影响，排除血清因子的协同作用的可能性。重组人IL-6、rhIL-11和rmGM-CSF仅在血清存在下与rmTPO有协同作用。rmIL-3和rmSCF可促进CFU-Mk的大集落和含有其它细胞群的混合型集落的增殖，提示这些细胞因子促进CFU-Mk未成熟细胞群的增殖。rmTpo促进rmGM-CSF或rmIL-3刺激的GM集落的生长和rhEpo刺激的红系爆发，有或没有rmIL-3。仅在血清或rmSCF存在下，rmTpo与rmIL-3协同作用也显著增加HPP集落。

项目成果

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