Characterization of a novel anti-ENA antibodies

新型抗 ENA 抗体的表征

• 批准号: 02670486
• 负责人: MIYAGAWA Sachiko
• 金额: $ 1.54万
• 依托单位: Nara Medical University (C)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670486/
• 关键词: antinuclear antibodies; extractable nuclear antigens; thrombocytopenic purpura; iopathic throbocyopenic purpura; antinuclear antibodies; antiーENA antibodies

Circulating antibodies against certain extractable nuclear antigens (ENA) have been shown to have diagnostic importance in connective tissue diseases. We reported an antibody against ENA found in the sera of patients with idiopathic thrombocytopenic purpura (ITP). The antigen, tentatively designated TPNA (thrombocytopenic purpura associated nuclear antigen) was distinct from UlRNP/Sm, SSA/SSB, Scl-70, PCNA, PM-Scl, Jo-1, and Ku by immunodiffusion. On immunoblotting, the anti-TPNA serum recognized polypeptides of 99 kd, 98 kd, and 96 kd in rabbit thymus extracts and a 99 kd polypeptide in KB cell extracts. The anti-TPNA antibody was detected in the sera of 2 out of 360 ANA positive patients, both children with clinical features of ITP. One patient developed systemic lupus erythematosus eight years after the onset of ITP.

针对某些可提取核抗原（ENA）的循环抗体已被证明在结缔组织疾病中具有诊断重要性。本文报道了在特发性血小板减少性紫癜（ITP）患者血清中发现的一种抗ENA抗体。经免疫扩散鉴定，该抗原与UlRNP/Sm、SSA/SSB、Scl-70、PCNA、PM-Scl、Jo-1、Ku等不同，暂定名为TPNA（血小板减少性紫癜相关核抗原）。在免疫印迹中，抗TPNA血清识别兔胸腺提取物中的99 kd、98 kd和96 kd多肽和KB细胞提取物中的99 kd多肽。在ANA阳性的360例患儿中，有2例患儿血清中检测到抗TPNA抗体，均为ITP患儿。1例患者在ITP发作8年后发生系统性红斑狼疮。

项目成果

Miyagawa S,Inagaki Y,Okada N,et al: "A novel antiーENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." Journal of Dermatology. 18. (1991)

Miyakawa S、Inagaki Y、Okada N 等人：“儿童特发性血小板减少性紫癜患者血清中的一种新型抗 ENA 抗体。”皮肤病学杂志 18。

Miyagawa S, Inagaki Y, Okasa N, et al: "A novel anti-ENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)

Miyakawa S、Inagaki Y、Okasa N 等人：“儿童特发性血小板减少性紫癜患者血清中的一种新型抗 ENA 抗体。”

Miyagawa S,Inagaki Y,Okada N,et al: "A novel anti-ENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)

Miyakawa S、Inagaki Y、Okada N 等人：“儿童特发性血小板减少性紫癜患者血清中的一种新型抗 ENA 抗体。”

Kurata Y, Miyagawa S, Kosugi S, et al: "Clinical significance of antinuelear antibodies in ITP" Jap J Clin Hematol.

Kurata Y、Miyakawa S、Kosugi S 等人：“ITP 中抗核抗体的临床意义”Jap J Clin Hematol。

Miyagawa S,Inagaki Y,Okada N,et al: "A novel antiーENA antibody in sera of patients with childhood idiopathic thrombocytopenic purpura." J Dermatol. 18. 69-73 (1991)

Miyakawa S、Inagaki Y、Okada N 等人：“儿童特发性血小板减少性紫癜患者血清中的一种新型抗 ENA 抗体。”J Dermatol。