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Connective tissue changes due to long-term ultraviolet exposure : Structural and biochemical studies

长期紫外线照射导致的结缔组织变化：结构和生化研究

基本信息

批准号：
02670485
负责人：
TSUJI Takuo
金额：
$ 1.47万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The difference in dermal connective tissue alteration between chronological aging and photoaging using human and animal skin has been disclosed.Materials and Methods. Human skin and animal skin was used. Human skin : sun or UV exposed and protected skin of healthy men(31 young and 32 old)and patients with psoriasis(2 young and one old, treated with PUVA). Animal skin : hairless rats skin(5 UVB(1 MED)exposed for 13 months and 5 control). Ultrasonic skin images were obtained from sun-exposed and protected areas of some healthy men.Skin biopsy specimens were obtained from all men and animals mentioned above, and were processed for light and electron microscopy, and for amino acid analysis for elastic fibers.Results. Ultrasonically, on the images, the dermis was clearly located between white line of the epidermis and underlying fatty tissue. The dermis was thinner in aged persons than young persons, and that of sun-exposed area wag thinner than that of protected area. - One can observe below the white line, a black zone showing a relatively homogeneous, non-echogenic structure in only sun-exposed area of aged persons.Histologically, in sun-exposed, PUVA-treated and UVB exposed skin, multiplication and down ward to the dermis of the basal lamina, irregularly arranged thickened collagen fibers, increased and thickened, various degrees of damaged elastic fibers, and amyloid deposits(in some healthy and PUVA-treated persons)were seen. In protected skin or control rat skin, a layer of basal lamina, regularly arranged, thin collagen fibers, thickened but not damaged elastic fibers, were seen. No amyloid deposits were seen.Analysis of amino acid in elastic fibers showed glutamic and asparatic acid, sreonine, serine, and lysine were higher in sun-exposed skin, while they were lower in protected skin when compared with that in young adults' skin.

已经公开了使用人和动物皮肤的时间老化和光老化之间的真皮结缔组织改变的差异。使用人皮和动物皮。人体皮肤：健康男性（31名年轻人和32名老年人）和银屑病患者（2名年轻人和1名老年人，用PUVA治疗）暴露于阳光或紫外线并受到保护的皮肤。动物皮肤：无毛大鼠皮肤（5只UVB（1 MED）暴露13个月，5只对照）。对部分健康男性的暴露区和保护区皮肤进行超声检查，对上述所有人和动物的皮肤活检标本进行光镜和电镜检查，并对弹性纤维进行氨基酸分析。超声检查，在图像上，真皮清楚地位于表皮的白色线和下面的脂肪组织之间。老年人真皮层薄于青年人，日光暴露区薄于防护区。- 在白色线以下可观察到一个黑色区域，显示仅在老年人的阳光暴露区域中的相对均匀的无回声结构。组织学上，在阳光暴露、PUVA处理和UVB暴露的皮肤中，基底膜增殖并向下延伸至真皮，胶原纤维不规则排列增厚，增加和增厚，弹性纤维不同程度受损，和淀粉样蛋白沉积（在一些健康和PUVA治疗的人）。在保护组和对照组大鼠皮肤中，可见一层排列规则的基底膜，胶原纤维较细，弹性纤维增厚但未受损。弹性纤维中氨基酸分析显示，与青年人皮肤相比，暴露于日光的皮肤中谷氨酸、谷氨酸、丝氨酸、赖氨酸含量较高，而保护皮肤中谷氨酸、谷氨酸、丝氨酸、赖氨酸含量较低。