Circulating suppressing factor for muscarinic acethylcholine receptor in patients with senile dementia of Alzheimer type

阿尔茨海默型老年痴呆患者毒蕈碱乙酰胆碱受体循环抑制因子

• 批准号: 02670514
• 负责人: MORIMOTO Shigeta
• 金额: $ 1.47万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670514/
• 关键词: Senile dementia; Senile dementia of Alzheimer type; Vascular-type dementia; Muscarinic acethylcholine receptor; Circulating suppressing factor; Familial Alzheimer disease; Pick病; 循環血液中因子

Alzheimer's disease is one of the main cause for senile dementia. In the present study, possible circulating factor, which affect the muscarinic acethylcholine receptor of the synaptic vesicle from the rat whole brain, was evaluated in the serum of 76 senile subjects (28 males and 48 females, mean <plus-minus>SD age of 78.5 <plus-minus> 8.6 years old). Their cognitive function was assessed by Mini-Mental State, and these subjects were divided into nondementic subjects with the score of 21 or more, and subjects with dementia with the score of 20 or less. The latter subjects were further divided into senile dementia with Alzheimer type (SDAT) and vascular type dementia (VD) using Hatchinski's ischemic score. The mean suppression rate of the serum from the SDAT patients on the binding of tritiated quinuclidinyl benzilate ([ ^3H]QNB), an antagonist for muscarinic acethylcholine receptor, to the rat synaptic membrane, being -13.3 <plus-minus> 6.1% of the control value, was significantly greater than that of the serum from the non-dementic subjects, being -8.9 <plus-minus> 5.0%. However, that in VD group, being -10.0 <plus-minus> 3.5%, was not significantly different from the control value. Moreover the suppression rate of the serum on the [ ^3H]QNB binding to the membrane was significantly positively correlated with the score for the Mini-Mental state (r = 0.480, p<0.01) in the SDAT group. These data support the hypothesis that circulating suppressing factor may participate in the pathogenesis of SDAT.

阿尔茨海默病是老年痴呆症的主要原因之一。在本研究中，对 76 名老年受试者（28 名男性和 48 名女性，平均±SD 年龄为 78.5±8.6 岁）的血清中评估了可能影响大鼠全脑突触小泡毒蕈碱乙酰胆碱受体的循环因子。通过简易精神状态评估他们的认知功能，将这些受试者分为21分或以上的非痴呆受试者和20分或以下的痴呆受试者。使用 Hatchinski 缺血评分将后者进一步分为阿尔茨海默型老年痴呆（SDAT）和血管型痴呆（VD）。 SDAT患者血清对毒蕈碱乙酰胆碱受体拮抗剂氚苯环奎宁环酯（[^3H]QNB）与大鼠突触膜结合的平均抑制率为-13.3±对照值的6.1%，显着高于非痴呆受试者血清的-8.9 <正负> 5.0%。然而，VD组为-10.0±3.5%，与对照值没有显着差异。此外，SDAT组中血清对[^3H]QNB与膜结合的抑制率与迷你精神状态评分显着正相关(r = 0.480，p<0.01)。这些数据支持循环抑制因子可能参与 SDAT 发病机制的假设。

项目成果

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“老年受试者和年轻受试者的成纤维细胞中对甲状旁腺激素反应产生的环 AMP 的比较。”

北野 昇一,森本 茂人,宮 敬子,白石 恒人,福尾 恵介,今中 俊爾,高 栄男,荻原 俊男: "老年期痴呆における流血中のムスカリン性アセチルコリン受容体抑制因子に関する研究" 日本老年医学会雑誌. 28. 345-350 (1991)

Shoichi Kitano、Shigeto Morimoto、Keiko Miya、Tsuneto Shiraishi、Keisuke Fukuo、Shunji Imanaka、Hideo Taka、Toshio Ogihara：“老年痴呆症血流中毒蕈碱乙酰胆碱受体抑制剂的研究”日本老年医学学会杂志。 ）

Kitano S;Morimoto S,Fukuo,K;Miya K;Yasuda O;Koh E;Hirotani A;Nakahashi T;Tamatani M and Ogihara T.: "Circulating suppressing factor for muscarinic acetylcholine receptor in patients with senile dementia of Alzheimer type" Gerontology. (1992)

Kitano S;Morimoto S,Fukuo,K;Miya K;Yasuda O;Koh E;Hirotani A;Nakahashi T;Tamatani M 和 Ogihara T.：“阿尔茨海默型老年性痴呆患者毒蕈碱乙酰胆碱受体的循环抑制因子”老年学

宮 敬子,森本 茂人,福尾 恵介,今中 俊爾,白石 恒人,山本 秀樹,北野 昇一,宮下 善行,井上 卓夫,廣谷 淳,荻原 俊男: "老年女性における知的機能へのカルシウム関連因子の関与について" 日本老年医学会雑誌. 28. 34-39 (1991)

Keiko Miya、Shigeto Morimoto、Keisuke Fukuo、Shunji Imanaka、Tsuneto Shiraishi、Hideki Yamamoto、Shoichi Kitano、Yoshiyuki Miyashita、Takuo Inoue、Jun Hirotani、Toshio Ogihara：“钙相关因素对老年女性智力功能的影响”“关于日本老年医学会杂志. 28. 34-39 (1991)

Morimoto S,Masugi F,Hironaka T,Shiraishi T,Itoh K,Yamamoto H,Koh E,Onishi T and Kumahara Y.: "Relation of serum parathyroid hormone to cognitive functio in elderly females." In:New Actions of Parathyroid Hormone Plenum Publishing Corporation.Press,New Yor

Morimoto S、Masugi F、Hironaka T、Shiraishi T、Itoh K、Yamamoto H、Koh E、Onishi T 和 Kumahara Y.：“老年女性血清甲状旁腺激素与认知功能的关系”。