Experimental Study of a Hybrid Artificial Esophagus Using Cultured Human Esophageal Epithelial Cells

利用培养的人食管上皮细胞进行混合人工食管的实验研究

• 批准号: 02670588
• 负责人: ANDO Nobutoshi
• 金额: $ 1.54万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670588/
• 关键词: Artificial Esophagus; Hybrid Organ; Esophageal Epithelial cell; Cell Culture; Collagen Gel; Transplantation; ハイブリッド型人工食道; 正常ヒト食道上皮細胞培養; コラーゲンゲル培養; 再構築型人工食道; 培養表皮細胞浮遊液; 細胞播種; 培養表皮細胞シ-ト; ディスバ-ゼ

It is important to cover the artificial esophagus with epithelium for preventing eventual stenosis and reinforcing the lumen. We examined possibility of epithelization of the surface of a latissimus dorsi muscle of athymic mice using cultured human esophageal epithelial cells.Normal human esophageal mucosa (0.5 cm2) were processed from the resected specimen of patients with esophageal cancer and epithelial cells were cultured on the collagen gel in culture dish (30 cm2) for about 10 days. The collagen sheets on which the cells were cultured were transplanted on the surface of a latissimus dorsi muscle of athymic mice. They were sacrificed 4, , 12 and 16 days after transplantation and the transplanted collagen sheets were studied histologically.On the 4th day after transplantation, the epithelial cell layer of the collagen sheet was confirmed to be kept intact and the connective tissue was observed to infiltrate into the collagen layer. On the 16th day, the vascularization in the collagen layer and many layers of mature epithelization were observed. We succeeded in transplantation of cultured human esophageal cells onto the muscle of athymic mice by no later than the 16th day after surgery.This study suggests that epithelization on the lining of the latissimus dorsi muscle tube is possible using cultured human esophageal epithelial cells.

用上皮覆盖人工食管对于防止最终狭窄和加强管腔是重要的。我们用培养的人食管上皮细胞检测了无胸腺小鼠背阔肌表面上皮化的可能性。从食管癌患者切除的标本中处理正常人食管粘膜（0.5cm 2），并将上皮细胞在培养皿（30cm 2）中的胶原凝胶上培养约10天。将其上培养有细胞的胶原片移植到无胸腺小鼠的背阔肌表面。分别于移植后4、12、16天处死动物，对移植的胶原片进行组织学观察，移植后4天，胶原片上皮细胞层完整，可见结缔组织浸润胶原层。第16天，胶原层内可见血管形成，并有多层成熟上皮形成。我们成功地将培养的人食管上皮细胞移植到无胸腺小鼠的肌肉上，不迟于手术后16天。这项研究表明，使用培养的人食管上皮细胞在背阔肌肌管的衬里上上皮化是可能的。

项目成果

佐藤 道夫: "培養ヒト食道上皮細胞を用いたハイブリッド型人工食道開発における基礎的研究" PROGRESS IN MEDICINE. (1993)

佐藤道雄：“利用培养的人食管上皮细胞开发混合人工食管的基础研究”医学进展（1993）。

佐藤 道夫: "ハイブリット型人工食道内腔上皮化のための基礎的研究" PROGRESS IN MEDICINE. (1992)

佐藤道雄：“混合人工食管腔上皮化的基础研究”医学进展（1992）。

長島敦: "培養表皮細胞を応用した人工食道開発に関する実験的研究" 人工臓器. 20. 631-634 (1991)

Atsushi Nagashima：“利用培养表皮细胞开发人工食道的实验研究”人工器官。20. 631-634 (1991)

佐藤 道夫: "ハイブリッド型人工食道内腔上皮化のための基礎的研究" PROGRESS IN MEDICINE. 11. 2851-2852 (1992)

佐藤道雄：“混合人工食管腔上皮化的基础研究”医学进展 11. 2851-2852 (1992)。

Michio Sato: "Experimental Study of a Hybrid Artificial Esophagus Using Cultured Human Esophageal Epithelial cells" ASAIO Journal (July/September). (1993)

Michio Sato：“使用培养的人类食管上皮细胞进行混合人工食管的实验研究”ASAIO 杂志（7 月/9 月）。