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Tissue Engineered Neoesophagus

组织工程新食道

基本信息

批准号：
17591440
负责人：
ANDO Nobutoshi
金额：
$ 2.31万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591440/
关键词：
implantation regenerative medicine cell culture biotechnology

项目摘要

1.Isolalon and culture of rat's esophageal keratinocytes1) Donor rats were sacrifioed and pieces of esophageal mucosa were obtained. They were incubated in dispase solution and then stirred in trypsin solution. Rat's esophageal keratinocytes were successfully harvested.2) Esophageal keratiocyies were successfully cultured by 3T3 feeder method on collagen gel.2.Implantation of the esophageal keratinocytes sheet to the omentun or to the latissimus dorsi muscle and regeneration of neoesophagus1) The collagen sheets with cuttured keratinocytes were implanted in recipient rat's latissimus dorsi muscle or in omentun and rolled and sutured. One 2, 3, week after implantation the tissue were taken and examined. They formed tube and neoesophagi were confirmed to regenerate macroscopically and microscopically.3. Anastomosis between Neoesophagus and small intestine and replacement of the cervical esophagus with neoesophagus1)Neoesophagus male in omentum were anastomosed to native jejunum in an end … More to end fashion. These rats also received Roux-Y operation and anastomoses were kept from food passage. Operated rats were all survived and the anastomoses were successfully made. Mucosa of neoesophagi were maintained and microscopically grew and became simliar to normal esophageal mucosa.2) Neoesophagus made in latissimus dorsi muscle was pulled up to neck at the time of implantation. One week after implantation the 1cm cervical esophagus of recipient rat were resected and replaced with neoesophagus and anastomosed them an end to end fashion. The rats survived 6 days after the operation and anastomoses were successfully made.4. Human esophageal keratinocyte culture and implantation to nude mice1) Normal human esophageal pieces of mucosa were obtained from esophageal cancer patients and keratinocytes were harvested as above method. They were cultured on collagen gel with serum free medium.2) The collagen sheets were implanted on latissimus dorsi muscle of nude mice. By 16 days the epithetial cel layer was similar to normal human esophageal epithetum. Less

1.大鼠食管角朊细胞的分离与培养1）取供体大鼠，取食管黏膜组织块。将它们在分散酶溶液中孵育，然后在胰蛋白酶溶液中搅拌。2.食管角化细胞片移植于大网膜或背阔肌与新生食管的再生1）将带角化细胞的胶原片移植于受体大鼠背阔肌或大网膜内，卷起缝合。植入后2、3周取组织进行检查。大体和镜下均证实它们形成管状和新食管再生.新生食管与小肠吻合及代颈段食管术1）将大网膜内新生食管与自体空肠吻合 ...更多信息来终结时尚这些大鼠也接受Roux-Y手术，并禁止进食。手术大鼠全部存活，造模成功。2）在背阔肌内制作的新食管在植入时被向上牵拉至颈部。植入后1周，切除受体大鼠颈段1cm食管，代之以新食管，端端吻合。大鼠术后6天存活，成功制作成骨瓣.人食管角朊细胞培养及裸鼠移植瘤的研究1）取食管癌患者正常食管粘膜组织块，按上述方法分离角朊细胞。2）将胶原片移植于裸鼠背阔肌上。到16天，上皮细胞层与正常人食管上皮相似。少