Experimental Study for Development of Artificial Esophagus

人工食管研制的实验研究

• 批准号: 60570628
• 负责人: ANDO Nobutoshi
• 金额: $ 1.02万
• 依托单位: Department of Surgery, School of Medicine, Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570628/
• 关键词: Artificial Esophagus; Cell-Seeding; 上皮増殖因子(EGF)

The problems as for the clinical application of artificial esophagus are the anastomotic insufficiency and the stenosis. To resolve these points and to develope the practical artificial esophagus, we have searched for the materials for the prothesis which suit to the body, and have researched about the rapid epithelization onto the prothesis.As the materials of the prothesis, a tube made of absorbable suture thread (polyglycoric acid) and a stainless mesh tube were tried in dog. These were wrapped with omentum and buried in the subcutaneous tissue. Four weeks later, tube-formed granulation was found inside the indwelled tube. Same kind of granulation was also formed when the muscle flap of M. latissumus dorsi was used for wrapping. We have purposely inserted the edge of the skin flap into the prothesis-to be. From this inserted skin edge, squamous cell epithelium has expanded. However, this epithelization is slow to spread all over the granulation. So controlling infection, we are trying the cell-seeding and applying EGF (epidermal growth factor) for the rapid epithelization over the granulation inside the prothesis-to-be.

人工食管临床应用中存在的问题是吻合口不全和狭窄。为了解决这些问题，开发实用的人工食道，我们寻找适合人体的假体材料，并对假体快速上皮化进行了研究。采用可吸收缝线（聚甘酸）和不锈钢网管作为假体的材料，在犬身上进行了试验。它们被网膜包裹并埋在皮下组织中。四周后，留置管内可见管状肉芽。背阔肌瓣包绕时也可形成类似的肉芽。我们特意把皮瓣的边缘插入假体中。从插入的皮肤边缘，鳞状细胞上皮扩大。然而，这种上皮扩散到整个肉芽的速度很慢。因此，为了控制感染，我们正在尝试细胞播种并应用表皮生长因子（EGF）在假体内部的肉芽上快速形成上皮。

项目成果

池端幸彦: 最新医学. 41. 2188-2190 (1986)

池端幸彦：最新医学。41。2188-2190（1986）

Yukihiko, Ikehata: "Experimental Study for Epithelization of Artificial Esophagus" Saishin-Igaku. 41. 2188-2190 (1986)

Yukihiko，Ikehata：“人工食管上皮化的实验研究”Saishin-Igaku。