Experimental Study of Lung Preservation -from the viewpoint of the changes of anionic sites

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We studied the changes of anionic sites produced by preservations in basement membrane of lungs using Wister rats.They were divided into 4 groups as follows ;A group : controlB group : stored at room temperature in heparinized salineC group : stored at 37ﾟC in heparinized salineD group : stored at 37ﾟC in EP3 solutionSmall tissue blocks were taken from the lungs and anionic sites were stained using strong cationic polyethyleneimine as a tracer following Schurer's method. After staining of anionic sites, we observed the sections on. electrone microscopy and caliculated the dot density of basement membrenes(dot number / cm at the magnification of 15410)and analyzed them by-the system(Hard : NEC 9801 VX, Soft : Nikon Cosmozone).RESULTSA group : 10.2<plus-minus>1B group : 12.6<plus-minus>1C group : 12.0<plus-minus>1D group : 11.8<plus-minus>1.8 (mean<plus-minus>S.CONCLUSIONCompared to the A group(control), B, C and D group hdd higher density of anionic sit significantly(p<0.05)We need more experiments to put an exact physiological interpretation on these results.