Experimental Study of Lung Preservation -from the viewpoint of the changes of anionic sites

肺保存的实验研究——从阴离子位点变化的角度

• 批准号: 02670612
• 负责人: KANKI Yutaka
• 金额: $ 1.47万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670612/
• 关键词: anionic sites; polyethyleneimine; lung preservation; 肺保存; heparan Sulfate proteoglycan; permeability

We studied the changes of anionic sites produced by preservations in basement membrane of lungs using Wister rats.They were divided into 4 groups as follows ;A group : controlB group : stored at room temperature in heparinized salineC group : stored at 37ﾟC in heparinized salineD group : stored at 37ﾟC in EP3 solutionSmall tissue blocks were taken from the lungs and anionic sites were stained using strong cationic polyethyleneimine as a tracer following Schurer's method. After staining of anionic sites, we observed the sections on. electrone microscopy and caliculated the dot density of basement membrenes(dot number / cm at the magnification of 15410)and analyzed them by-the system(Hard : NEC 9801 VX, Soft : Nikon Cosmozone).RESULTSA group : 10.2<plus-minus>1B group : 12.6<plus-minus>1C group : 12.0<plus-minus>1D group : 11.8<plus-minus>1.8 (mean<plus-minus>S.CONCLUSIONCompared to the A group(control), B, C and D group hdd higher density of anionic sit significantly(p<0.05)We need more experiments to put an exact physiological interpretation on these results.

本实验采用Wister大鼠，观察了肺基底膜内阴离子位点的变化，实验分4组，A组：对照组，B组：室温肝素化生理盐水保存组，C组：37 ℃肝素化生理盐水保存组，D组：从肺中取出小的组织块，用强阳离子聚乙烯亚胺作为示踪剂对阴离子位点进行染色，Schurer的方法。阴离子位点染色后，在电镜下观察，计算基底膜的点密度（15410倍下点数/ cm），并用NEC 9801 VX系统（硬）和NikonCosmozone系统（软）进行分析，结果表明：与A组（对照组）相比，B组：<plus-minus>10.21B组：<plus-minus>12.61C组：<plus-minus>12.01D组：<plus-minus>11.81.8（均数<plus-minus>S）。C、D组阴离子位点密度显著高于对照组（P&lt;0.05）。