Experimental Study of Lung Preservation -from the viewpoint of the changes of anionic sites

我们用Wister大鼠研究了保存液在肺基底膜中产生的阴离子位点的变化。将其分为4组：A组：对照组b组：肝素化盐室温保存C组：肝素化盐37ºC保存EP3 37ºC保存从肺中取小块组织，用强阳离子聚乙烯亚胺作为示踪剂按照Schurer’s法对阴离子部位进行染色。阴离子位点染色后，我们观察。在15410倍率下计算基底膜的网点密度（网点数/ cm），并通过系统（硬：NEC 9801 VX，软：Nikon Cosmozone）进行分析。结果tsa组：10.2<正负>1B组：12.6<正负>1C组：12.0<正负>1D组：11.8<正负>1.8（平均<正负>S）；结论与A组（对照组）相比，B、C、D组阴离子离子密度显著增高（p<0.05），需要更多的实验对这一结果进行准确的生理解释。

We studied the changes of anionic sites produced by preservations in basement membrane of lungs using Wister rats.They were divided into 4 groups as follows ;A group : controlB group : stored at room temperature in heparinized salineC group : stored at 37ﾟC in heparinized salineD group : stored at 37ﾟC in EP3 solutionSmall tissue blocks were taken from the lungs and anionic sites were stained using strong cationic polyethyleneimine as a tracer following Schurer's method. After staining of anionic sites, we observed the sections on. electrone microscopy and caliculated the dot density of basement membrenes(dot number / cm at the magnification of 15410)and analyzed them by-the system(Hard : NEC 9801 VX, Soft : Nikon Cosmozone).RESULTSA group : 10.2<plus-minus>1B group : 12.6<plus-minus>1C group : 12.0<plus-minus>1D group : 11.8<plus-minus>1.8 (mean<plus-minus>S.CONCLUSIONCompared to the A group(control), B, C and D group hdd higher density of anionic sit significantly(p<0.05)We need more experiments to put an exact physiological interpretation on these results.