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A Basic Research of Small Caliber Vascular Xenografts with Antithrombogenicity

小口径血管异种移植物抗血栓形成的基础研究

基本信息

批准号：
63570663
负责人：
KANKI Yutaka
金额：
$ 1.34万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

[Immunogenicity] To evaluate the effects of polyepoxy compounds (PC) on the immunogenicity of biomaterials, DDY mice were immunized with homogenates of glutaraldehyde(GA), glycerol polyglycidyl ether(GPE), and untreated dog (donor) arteries. Using their sera, donor peripheral lymphocytes were stained with FITC labeled anti-mouse IgG antibodies and analyzed with cytofluorometry. This experiment revealed that GPE reduced the immunogenicity of arteries to the same level as GA. To investigate the relationship between reaction time and immunogenicity, dog arteries were cross-linked with GPE for 1, 12, and 48 hourg. Using these treated arteries, the immunogenicity was detected. This experiment indicated thatcross-linking with GPE for 48 hours seemed to be the best treatment.[Mechanical properties] To evaluate the relationship between the reaction time and mechanical properties, dog arteries treated with GPE forl, 12, and 48 hours. A stress-strain (S-S) curve of each sample was recorded. From … More the S-S curve, the maximum stressforce of each sample was calculated and flexibility was compared using finite deformation theory. The results of this experiment showed that GPE made the arteries stronger and harder in proportion of reaction time. Bovine arteries were treated with GPE and GA for 48 hours to compare their effects on the mechanical properties.This experiment revealed that arteries treated with GPE for 48 hours were about 3 times as flexible as those treated with GA for 48 hours, despite a similar strength.[Antithrombogenicity] Protanine sulfate was used to increase heparin binding covalently to arteries with PC. Protamine sulfate solution was poured into dog arteries. Then the arteries were dipped into a PC solution to cross-link the protanine sulfate within the matrix of the arteries. Finally, these arteries were dipped into heparin solution. By this method, heparin was ionically bound to the inner side of the arteries. 80 dog arteries treated with this method were implanted to 40 dogs and 77 of 80 grafts remained patent. However, It takes more than 10 hours to heparinize the arteries. Therefore, we introduced a new method using GA instead of PC. Since it takes only 15 minutes to heparinaze the arteries using this method, we applied this new method to heparinize arteries after TEA clinically and met good results. Less

[免疫原性]为了评价聚环氧化合物（PC）对生物材料免疫原性的影响，用戊二醛（GA）、甘油聚缩水甘油醚（GPE）和未处理的狗（供体）动脉的匀浆免疫DDY小鼠。使用它们的血清，供体外周淋巴细胞用FITC标记的抗小鼠IgG抗体染色，并用细胞荧光测定法进行分析。本实验表明，GPE可将动脉的免疫原性降低至与GA相同的水平。为了研究反应时间和免疫原性之间的关系，将狗动脉与GPE交联1、12和48小时。使用这些处理的动脉，检测免疫原性。本实验表明，用GPE交联48小时似乎是最好的处理。[力学性能]为了评价反应时间与力学性能之间的关系，用GPE处理犬动脉1、12和48小时。记录每个样品的应力-应变（S-S）曲线。从 ...更多信息 S-S曲线，计算各试件的最大应力，并利用有限变形理论比较各试件的柔度。实验结果表明，GPE使动脉变得更强，更硬，与反应时间成正比。用GPE和GA处理牛动脉48小时，比较它们对力学性能的影响，实验表明，GPE处理48小时的动脉的柔韧性约为GA处理48小时的动脉的3倍，尽管强度相似。[抗血栓形成]使用硫酸鱼精蛋白增加肝素与PC的动脉共价结合。将硫酸鱼精蛋白溶液注入犬动脉中。然后将动脉浸入PC溶液中，以交联动脉基质内的硫酸鱼精蛋白。最后，将这些动脉浸入肝素溶液中。通过这种方法，肝素被离子结合到动脉的内侧。用这种方法处理的80条狗动脉植入40条狗，80条移植物中有77条保持通畅。然而，动脉肝素化需要10多个小时。因此，我们介绍了一种新的方法，使用遗传算法代替PC。由于该方法对动脉进行肝素化只需15分钟，我们将其应用于临床TEA后动脉的肝素化，取得了良好的效果。少