Role of Androgen Receptor in the Pathogenesis of Prostatic Cancer
雄激素受体在前列腺癌发病机制中的作用
基本信息
- 批准号:02670702
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The major purpose of this project had been the cloning of cDNA of androgen receptor at the start. Before cloning its cDNA by us. Chang. C and collaborators had succeeded in cloning and determined the sequence of cDNA of androgen receptor. So. we changed the strategy of out research and investigated the sugar chains in prostatic cancer by lectin histochemistry. especially peanut agglutinin(PNA). Cellular sugar chains play important roles in a number of cell functions, including cell-cell and cell-matrix interactions, cell surface receptors and cellular regulation. In addition to lectin histochemistry, DNA ploidy patterns and tumor volumes were analezed.Lectin histochemistry(PNA and Ulex-europaeus 1 : UEA-1) was examined in 33 patients with in cidental prostatic cancer, 41 with stage C and D, 10 with hormone refractory cancer and 24 with benign prostatic hyperplasia(BPH). PNA was reported that the expression of its binding sugar chain was suggested to be modulated by androgen levels. UEA … More -1 binding sugar chain was reported to be increased in adenocarcinomas of colon and rectum.The results were obtained as follows. 1)In BPH, the major binding sites of PNA were basal cells(75% of BPH-cases). UEA-1 staining were none to weak in columnar secretory cells.2)PNA binding was increased in incidental cancers(stage A1 and A2) cancers in comparison to BPH.In clinically advanced cancers(stage C and D), the intensity of PNA staining was weaker than in incidentalomas.3)UEA-1 binding was increased significantly in both incidental and advanced cancers more than in BPH.4)No relations were demonstrated between lectin bindings and cytological grade.5)The intensity of PNA and UEA-1 staining were strong in 80% and 100% of hormone refractory prostatic cancers, respectively.6)DNA ploidy patterns had no relations with the intensity of lectin histochemistry. In well-deferentiated incidentalomas, PNA binding sites in cancer cells were different according to DNA ploidy patterns. diploid cancer cells had PNA binding site in apical area, whereas non-diploid one had both in apical and supranuclear areas.7)The positive corelation was found between the intensity of UEA-1 staning and tumor volume in incidentalomas.Expression of PNA binding site, Which was reported to be modulated by androgen levels, was increased in incidentalomas more than BPH and clinically advanced cancers. This indicated that androgen and its cellular messengers including androgen receptor might play important roles in carcinogenesis of proatatic cancers. The different PNA binding site in well-differentiated cancer cells were observed according to DNA ploidy patterns. Although DNA ploidy patterns had no relations with the intensity of PNA staining, the qualitative and/or quantative alterations in of the enzymes involved in glycosylation were suggested to have relations to the abnormality in DNA ploidy.The increased UEA-1 binding sites in cancers and the positive corelation between the intensity of UEA-1 staning and tumor volume in incidentalomas indicated that the expression of UEA-1 binding sugar chains had close relation with tumor growth. Less
该项目最初的主要目的是克隆雄激素受体的基因。在我们克隆其cDNAs之前。张先生。C等人已成功克隆并测定了雄激素受体的基因序列。所以。我们改变了OUT研究的策略,用凝集素组织化学方法研究了前列腺癌中的糖链。尤其是花生凝集素(PNA)。细胞糖链在许多细胞功能中发挥着重要作用,包括细胞-细胞、细胞-基质相互作用、细胞表面受体和细胞调节。对33例前列腺癌、41例C、D期前列腺癌、10例激素难治性癌和24例良性前列腺增生症(BPH)进行了凝集素组织化学(PNA和Ulex-uropaeus 1:UEA-1)检测。据报道,PNA结合糖链的表达可能受雄激素水平的调节。UEA…据报道,结肠癌和直肠腺癌中有更多的结合糖链增加。1)在BPH中,PNA的主要结合部位是基底细胞(占BPH病例的75%)。2)偶发癌(A1期和A2期)PNA染色强度较偶发癌(C期和D期)弱。3)偶发癌和晚期癌UEA-1结合强度均显著高于BPH。4)凝集素结合与细胞学分级无关。5)PNA和UEA-1染色强度在激素非依赖性前列腺癌中分别为80%和100%。在分化良好的偶发瘤中,癌细胞中的PNA结合部位根据DNA倍体模式而不同。7)偶发瘤中UEA-1的表达强度与肿瘤体积呈正相关,偶发瘤中PNA结合部位的表达受雄激素水平的调节,在偶发瘤中的表达高于良性前列腺增生症和临床晚期癌。提示雄激素及其细胞信使,包括雄激素受体,可能在前列腺癌的发生发展中起重要作用。根据DNA倍体模式观察到高分化癌细胞中不同的PNA结合部位。DNA倍体类型与PNA染色强度无关,但糖基化相关酶的定性和/或定量改变可能与DNA倍体异常有关。肿瘤中UEA-1结合部位增多,偶发瘤中UEA-1结合强度与肿瘤体积呈正相关,提示UEA-1结合糖链的表达与肿瘤生长密切相关。较少
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Tomohiro Fujita, Hironobu Akino, Yusuke Kanimoto, and Kenichiro Okada, Taizou Shiraishi, Kazuya Nakakuki: "Study of incidental carcinoma of the prostate-II.Nuclear DNA analysis" Acta Urologica japonica. 37. 1009-1016 (1991)
Tomohiro Fujita、Hironobu Akino、Yusuke Kanimoto、Kenichiro Okada、Taizou Shiraishi、Kazuya Nakakuki:“前列腺偶发癌的研究-II.核 DNA 分析”Acta Urologica japonica。
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- 影响因子:0
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OKADA Kenichiro其他文献
OKADA Kenichiro的其他文献
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{{ truncateString('OKADA Kenichiro', 18)}}的其他基金
The effect of constitutional law between private subjects at Japanese and German courts in the early postwar years
战后初期日本和德国法院宪法对私人主体的影响
- 批准号:
15K16923 - 财政年份:2015
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
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