Analysis of structure of mucin type glycoprotein seaeted from endometrial Carcinoma

子宫内膜癌细胞产生高分子量粘蛋白型糖蛋白（MGP）。在子宫内膜癌患者的血清中检测到这种MGP。我们对子宫内膜癌细胞株分泌的MGP进行了结构分析，半纯化结果表明MGP为高分子物质，不含末端唾液酸。糖结构通过与先前定义的糖结构特异性反应的各种单克隆抗体进行分析，并且肽部分在通过高效液相色谱和免疫亲和柱进行高度纯化程序后通过Edman的氨基酸序列分析进行研究。MGP是由高度岩藻糖基化的聚乳糖胺碳水化合物结构和分子量约为100的核心肽组成。在EMBO数据库中没有发现同源肽结构，但推测的氨基酸序列与胰弹性蛋白酶III的DNA序列有很高的同源性，提示MGP与胰弹性蛋白酶III有部分同源基因。

Endometrial carcinoma cells produce a high molecular weight mucin type glycoprotein (MGP). This MGP was detected in sera of patients suffering from endometrial carcinoma. We investigated the structure of MGP secreted from endometrial carcinoma culture cell line.Semi-purification showed that MGP was high molecular material and there were no terminal sialic acids. The carbohydrate structure was analysed by various monoclonal antibodies which specifically react to previous defined carbohydrate structures, and peptide portion was investigated by Edman's amino sequence analysis after high purification procedures by high performance liquid chromatography and immunoaffinity column. It was elucidated that MGP was composed of highly fucosylated poly lactosamine carbohydrate structure and core peptide of approximately MW. 40K dalton.There were no homology peptide structures registered in peptides EMBO data base but the putative DNA sequence of the amino sequence had high homology with pancreatic elastase III.This results suggest that MGP partially share an ancient gene with pancreatic elastase III.