Vaginal infection with Ureaplasma urealyticum accounts for preterm delivery via induction of inflammatory responses

解脲支原体阴道感染通过诱导炎症反应导致早产

• 批准号: 17591765
• 负责人: TSUJI Yoshiyuki
• 金额: $ 2.35万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591765/
• 关键词: Mycoplasma; Ureaplasma; Abortion; Infertility; immunodeficiency; IL-8; TLR; IL-18

Ureaplasma urealyticum is a member of the family Mycoplasmataceae and often observed in the vagina of pregnant women. In this study, we examined if ureaplasmal infection is associated with preterm delivery and whether ureaplasmal lipoprotein (LP) has the capacity to stimulate monocytes to produce pro-inflammatory cytokines relevant to preterm delivery in vitro. We selected 100 cases of normal delivery and 45 cases of preterm delivery at random. We generated a monoclonal antibody (mAb) against U.urealyticum urease that selectively and positively stained it in vaginal secretions of infected women but not of uninfected women. The preterm delivery group showed significantly higher incidence for vaginal infection with this bacteria than the normal delivery group. Since LP of Mycoplasma has a potent biological activity, we extracted ureaplasmal LP. THP-1 cells, human monocytic cells, produced interleukin (IL)-8, a potent pro-inflammatory cytokine associated with preterm delivery, and showed apoptotic cell death in response to the LP. These results suggested that U. urealyticum infection might play a causative role in preterm delivery via LP-induced of IL-8 production and apoptosis.

解脲支原体是支原体科的一员，常见于孕妇阴道。在这项研究中，我们研究了脲原体感染是否与早产有关，以及脲原体脂蛋白（LP）是否有能力刺激单核细胞产生与早产相关的促炎细胞因子。我们随机选择100例正常分娩和45例早产。我们制备了一种针对解脲脲酶的单克隆抗体（mAb），该抗体在感染妇女的阴道分泌物中选择性地呈阳性染色，而在未感染妇女的阴道分泌物中则没有。早产组阴道感染这种细菌的发生率明显高于正常分娩组。由于支原体LP具有很强的生物活性，我们提取了脲原体LP。THP-1细胞，人类单核细胞，产生白细胞介素(IL)-8，一种与早产相关的强效促炎细胞因子，并在LP反应中显示凋亡细胞死亡。这些结果提示解脲菌感染可能通过lp诱导IL-8的产生和细胞凋亡而导致早产。

项目成果

Vaginal infection with Ureaplasma urealyticum accouts for prererm delivery via induction of inflammatory responses

解脲支原体阴道感染通过诱导炎症反应促进预产

• 发表时间: 2008
• 期刊: Microbiology and Immunlolgy (In press)
• 作者: Kayoko Harada;Hiroyuki Tanaka;Shinji Komori;Yoshiyuki Tsuji;Kumiko Nagata;Hiroko Tsutsui;Koji Koyama
• 通讯作者: Koji Koyama

Analysis of the relationship between ureaplasmal infection and premature birth

解脲支原体感染与早产的关系分析

• 发表时间: 2007
• 作者: Kayoko Harada;Hiroyuki Tanaka;Shinji Komori;Yoshiyuki Tsuji;Kumiko Nagata;Hiroko Tsutsui;Koji Koyama;原田 佳世子;原田 佳世子;原田 佳世子;原田 佳世子;Kayoko Harada
• 通讯作者: Kayoko Harada

Ureaplasma感染と早期産の関連およびUreaplasma由来リポ蛋白の作用の検討

解脲支原体感染与早产的关系及解脲支原体脂蛋白的影响研究

• 发表时间: 2007
• 作者: Kayoko Harada;Hiroyuki Tanaka;Shinji Komori;Yoshiyuki Tsuji;Kumiko Nagata;Hiroko Tsutsui;Koji Koyama;原田 佳世子;原田 佳世子;原田 佳世子
• 通讯作者: 原田 佳世子

ウレアプラズマと早産の関連ならびにウレアプラズマリポ蛋白による単球細胞でのTLRの誘導

解脲支原体与早产的关系及解脲支原体脂蛋白诱导单核细胞TLR

• 发表时间: 2007
• 作者: Kayoko Harada;Hiroyuki Tanaka;Shinji Komori;Yoshiyuki Tsuji;Kumiko Nagata;Hiroko Tsutsui;Koji Koyama;原田 佳世子
• 通讯作者: 原田 佳世子

Analysis of the relationship between ureaplasmal infection and premature birth.

解脲支原体感染与早产的关系分析

• 发表时间: 2007
• 作者: Harada;K.;Tanaka;H.;Tsuji;H.;Koyama
• 通讯作者: Koyama