INVESTIGATION FOR IMMUNOGENETIC BACKGROUND OF ANTISPERM ANTIBODY,INVOLVEMENT OF HLA-II

对36例血清中含有精子固定抗体的妇女，采用外周血白细胞DNA检测其HLA-DR和DQ型。采用PCR-SSO、PCR-SSOP和PCR-RFLP对HLA-DR和DQ位点进行分型。与日本正常人群（HLA-DRB1的单倍型为986，HLA-DQA1的单倍型为1068，HLA-DQB1的单倍型为2432）相比，估计患者的单倍型频率具有统计学意义。HLA-DRB1和HLA-DQB1位点的表型显著升高，但HLA-DQA1位点的表型无显著升高。HLA-DRB1**0901的基因频率为25%，而正常人群为13.6% (p<0.0085)； HLA-DRB1**1501的基因频率为12.5%，而正常人群为6.7% (p<0.0025)； HLA-DQB1**0602的基因频率为13.9%，而正常人群为6.8% (p<0.0089)； HLA-DQB1**0303的基因频率为25%，而正常人群为14.8% （p<0.0018）。HLA-DRB1**0901-DQB1**0303单倍型在精子固定抗体的不育女性中不平衡最强。HLA-DRB1**0901-DQB1**0303的这种单倍型在白种人中非常罕见（单倍型频率从0到几个百分点），但在日本人中却非常高（约占13%）。日本患者HLA-DRB1**0901-DQB1**0303的高频率可能是精子固定抗体出现频率高于其他国家报道的原因。说明人的抗精子免疫受HLA-DR或DQ基因的制约和控制。

Thirty six women, possessing of sperm immobilizing antibody in their sera were examined of their HLA-DR and DQ types using DNA obtained from peripheral blood white cells.The typing of HLA-DR and DQ locus were performed by PCR-SSO,PCR-SSOP and PCR-RFLP.The determined haplotype frequencies of patients were estimated of statistical significance, compared to normal Japanese populations (986 haplotype for HLA-DRB1,1068 for HLA-DQA1 and 2432 for HLA-DQB1). There were significant increase in phenotypes of HLA-DRB1 and HLA-DQB1 locus but not in HLA-DQA1 locus. There were 25 gene frequency percent for HLA-DRB1**0901 in contrast to 13.6 percent in normal population (p<0.0085) , 12.5 percent of patients of HLA-DRB1**1501 in contrast to 6.7 percent of normal population (p<0.0025) , 13.9 percent in contrast to 6.8 percent of HLA-DQB1**0602 (p<0.0089) and 25 percent of patients in contrast to 14.8 percent of normal population of HLA-DQB1**0303 (p<0.0018). The most strong disequilibrium found in HLA-DRB1**0901-DQB1**0303 haplotype among infertile women possessing sperm immobilizing antibody. This haplotype of HLA-DRB1**0901-DQB1**0303 is very rare in Caucasians (from 0 to several percent of haplotype frequency) but characteristic high in Japanese (about 13 percent of that). The high frequency of HLA-DRB1**0901-DQB1**0303 among the patients may account for higher frequency of sperm immobilizing antibody in Japan compared with another country reports.It was elucidated that anti-sperm immunity of human was restricted and controlled by HLA-DR or DQ genes.