IMMUNOPATHOLOGY IN THE EYES WITH EXPERIMENTAL UVEITIS

实验性葡萄膜炎眼部的免疫病理学

• 批准号: 3856041
• 负责人: C-C CHAN
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3856041
• 关键词: MHC class II antigen; antigen presenting cell; antiinflammatory agents; autoimmune disorder; cellular immunity; disease /disorder model; endotoxins; eye pharmacology; fibroblasts; helper T lymphocyte; immunochemistry; immunocytochemistry; immunomodulators; immunopathology; immunopharmacology; inflammation; laboratory mouse; laboratory rat; leukocyte activation /transformation; major histocompatibility complex; phospholipase A2; phospholipase inhibitor; retinal pigment epithelium; surface antigens; synthetic peptide; uveitis

The identity and topographic localization of immunocompetent cells and the alteration of surface markers on ocular resident cells in mice and rodents with experimental autoimmune uveoretinitis (EAU) were analyzed by immunohistochemical studies and in situ hybridization. T lymphocytes were the predominantly infiltrating cells in rat EAU, yet both macrophages and T helpers/inducers were the predominant infiltrating cells in mouse EAU. T lymphocyte specificity is directed to small fragments of antigen bound to cell surface major histocompability complex (MHC) molecules, which are presented on the surface of specialized antigen-presenting cells. The expression of MHC class II antigens was observed on ocular resident cells such as retinal pigment epithelium (RPE), retinal endothelium, keratocytes, fibroblasts, and ciliary epithelium in rodents. The expression of MHC class II antigens is confined to ocular resident cells immediately at the inflammatory sites in mouse EAU, which was characterized by focal lesions and chronicity. Both the infiltrating cell subpopulation and the expression of class II antigens on ocular resident cells can be altered by various immunomodulating agents. Experimental endotoxin-induced uveitis (EIU) is a model for anterior uveitis in humans. The mechanism of this inflammation is still unclear, although activation of phospholipase A2 (PLA2) may play a role in the initiation and propagation of this disease. Study of the effect of a novel synthetic peptide and a PLA2 inhibitor, antiflammin, showed inhibition of EIU in rats. The particular vascular structure of the anterior uvea and the role of adhesion molecules (such as intercellular adhesion molecule and endothelial leukocyte adhesion molecule) and their ligand, (lymphocyte function-associated antigen) were also examined in EIU and EAU models.

免疫活性细胞的身份和拓扑定位以及免疫活性细胞的功能。 小鼠和啮齿类动物眼常驻细胞表面标志物的改变 实验性自身免疫性葡萄膜视网膜炎（EAU）进行了分析， 免疫组化和原位杂交。T淋巴细胞 大鼠EAU中主要浸润细胞，但巨噬细胞和 T辅助细胞/诱导细胞是小鼠EAU中主要的浸润细胞。不 淋巴细胞特异性针对结合于 细胞表面主要组织相容性复合体（MHC）分子， 在专门的抗原呈递细胞的表面上呈递。的 在眼驻留细胞上观察到MHCII类抗原的表达 如视网膜色素上皮（RPE）、视网膜内皮、角膜细胞 成纤维细胞和睫状上皮。MHC类分子的表达 II型抗原仅限于眼驻留细胞， 小鼠EAU中的炎症部位，其特征在于局灶性病变 和慢性病。浸润细胞亚群和表达 眼驻留细胞上的II类抗原的量可以通过各种方法改变。 免疫调节剂。 实验性内毒素诱导的葡萄膜炎（EIU）是一种模型， 人类的前葡萄膜炎。这种炎症的机制仍然是 尽管磷脂酶A2（PLA 2）的激活可能在 这种疾病的发生和传播。研究A的影响 新的合成肽和PLA 2抑制剂antiflammin显示， 抑制大鼠EIU。这种特殊的血管结构 前葡萄膜和粘附分子（如细胞间粘附分子）的作用 粘附分子和内皮白细胞粘附分子）及其 EIU中还检测了淋巴细胞功能相关抗原（ligand） EAU模型