Suppression of corneal graft rejection in rabbits by local administration of immunosuppressive agents.

局部给予免疫抑制剂抑制兔角膜移植排斥反应。

• 批准号: 02670795
• 负责人: KANAI Atsushi
• 金额: $ 1.47万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670795/
• 关键词: cyclosporin eye drops; FK-506 eye drops; FK-506 subconjunctival injection; corneal grafting; immunosuppressive effect; 角膜移植

Corneal graft rejection is one of the leading causes of failure in corneal graft surgery. The purpose of our research is to find the efficacy on the suppression of corneal graft rejection in rabbits by local administration of cyclosporin (CYA) and FK - 506. Before we study the experimental corneal grafting of the rabbits, we measured the concentration of the two drugs in the cornea after 50 mul single application. The concentration of CYA in the cornea reached 13OOng/gr at 30 minutes and 7OOng/gr at 24 hours after the instillation. On the other hand, the concentration of the FK - 506 in the cornea reached 370ng/gr at 30 minutes and 130ng/gr at 24 hours after the instillation.Fifty animals received corneal allografts and skin grafts were also exchanged between pairs 14 days after the keratoplasty. Group I (10 eyes) was 0.025% CYA eye drops, Group II (10 eyes) was 0.05 % CYA eye drops, Group III (10 eyes) was 0.1 % FK - 506, eye drops, Group IV (10 eyes) was FK-506 (0.1mg/kg) subconjunctival injection (once a week), Group V was control (no medication). All eyes were give 4times a day during 50 days after the surgery, graft rejection was found 4 of 10 eyes in Group I, 2 of 10 eyes in Group II, none of 10 eyes in Group III, one'of 10 eyes in Group IV and 8 of 10 eyes in control. 0.05% CYA eye drops, 0.1 % FK - 506 eye drops and FK - 506 subconjunctival injection were significantly suppressed the corneal graft rejection.

角膜移植排斥反应是角膜移植手术失败的主要原因之一。本研究旨在探讨局部应用环孢素(CyA)和FK-506对兔角膜移植排斥反应的抑制作用。在对兔实验性角膜移植进行研究之前，我们测量了两种药物单次给药50次后在角膜中的浓度。角膜中CyA浓度在滴眼后30分钟达130 ng/gR，24小时达70 ng/gR。另一方面，FK-506在角膜中的浓度在注射后30分钟达到370 ng/gR，在24小时达到130 ng/gr。I组(0.025%环磷酰胺滴眼液)10眼，II组(0.05%环磷酰胺滴眼液)，III组(0.1%FK-506滴眼液)，IV组(FK-506 0.1 mg/kg结膜下注射，每周1次)，V组为对照组。术后50d内每日给药4次，移植排斥反应：I组4只眼，II组2只眼，III组10只眼无一例，IV组1只眼，对照组8只眼。0.05%环磷酰胺滴眼液、0.1%FK-506滴眼液和FK-506结膜下注射均能明显抑制角膜移植排斥反应。

项目成果

Kobayashi, C. et al: "・Effect of FK-506 on corneal Allograft Survival in the Rabbit" J. Eye (Atarashii Ganka). 8(11). 1771-1774 (1991)

Kobayashi, C. 等人：“FK-506 对兔子角膜同种异体移植物存活的影响”J. Eye (Atarashii Ganka) 8(11) (1991)。

Kanai,A.,Alba,R.M.et al: "Effect of Cyclosporine eye drops on the survival of corneal grafts in rabbit" XII Transactions of the AsiaーPacific Academy of Ophthalmology. 467-470 (1990)

Kanai, A., Alba, R.M. 等人：“环孢素滴眼液对兔角膜移植物存活的影响”，亚太眼科学会 XII 刊，467-470 (1990)。

秋山 修一,横山 利幸,小林 千博,金井 淳,加賀山 彰: "FKー506点眼の角膜・前房内移行" あたらしい眼科. 8. 1445-1448 (1991)

Shuichi Akiyama、Toshiyuki Yokoyama、Chihiro Kobayashi、Jun Kanai、Akira Kagayama：“FK-506 滴眼液迁移到角膜和前房”《新眼科学》8. 1445-1448 (1991)。

Kanai, A., Kobayashi, C. et al: "・Effect of cyclosporin eye drops on the survival of corneal grafts in rabbit" VII Transaction of the Asia-Pacific Academy of Ophthalmology.

Kanai, A.、Kobayashi, C. 等人：“·环孢素滴眼液对兔角膜移植物存活的影响” VII 亚太眼科学会会刊。