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A morphological study on the innervation of the larynx and on the degeneration and regeneration of the neuromuscular junction in the denervated laryngeal muscle

喉部神经支配及失神经喉肌神经肌肉接头变性与再生的形态学研究

基本信息

批准号：
02670776
负责人：
YOSHIHARA Toshio
金额：
$ 1.34万
依托单位：
Tokyo Women's Medical College, Faculty of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670776/
关键词：
Intrinsic laryngeal muscle Neuromuscular junction electronmicroscopy Histochemistry Recurrent nerve palsy

项目摘要

The purpose of this study is to demonstrate the morphological and histochemical characteristics of the neuromuscular junctions (NMJ) in the denervated and reinnervated laryngeal muscles. The posteriorcricoarytenoid muscles of the adult cats were used. The specimens were examined by electromicroscopy and various histochemical procedures such as acetylcholinesterase (AchE) reaction, . 5-hydroxydopamine (5-OHDA) injection and calcitonin-gene related peptide (CGRP) antibody.Twenty-four hours after the transection of the left recurrent laryngeal nerve (RLN), no morphological changes were observed at the NMJ. Two days after denervation nerve terminals disappeared at the NMJ, and Scwann's cells covered the former junctional site (FJS). Six weeks after denervation the primary synaptic clefts were narrowed and flattened. The secondary synaptic clefts were still preserved. Nine weeks after denervation nerve terminals resembling autonomic nerves were observed at the FJS. Some of these terminals w … More ere labeled by 5-OHDA. Furthermre, the autonomic nerves around the vessels and the Schwann's tunnels increased. These findings indicate the newly denervated nerve fibers are adrenergic, and the autonomic nerves extend to the FJS and formate the synaptic contacts.Although ultrastructural changes were remarkable after denervation, AchE activity was relatively stable at the FJS for a long period. To examine the NMJ in the reinnervated laryngeal muscles, 'end to end' sutures of the nerves were done just after the transection of the left RLN. Two weeks after sutures the nerve terminals were not observed at the FJS yet. After three weeks, the newly formated nerve terminals were found in one-thirds of the FJS.It is well established that the neurotransmitter of the motor nerve fiber is Ach. The present study showed that CGRP immunoreactivity was demonstrated in the nerve terminals electronmicroscopically. These results suggest that CGRP is involved in the regulation of the complex contractile functions of the intrinsic laryngeal muscles. Less

本研究的目的是证明神经肌肉接头（NMJ）的形态学和组织化学特征在失神经支配和神经支配的喉肌。使用成年猫的环杓后肌。标本经电镜及乙酰胆碱酯酶（AchE）反应等多种组织化学方法检测。5-切断左侧喉返神经（RLN）后24 h，NMJ处未见形态学改变。失神经支配后2天，NMJ处神经终末消失，Scwann细胞覆盖前交界处（FJS）。失神经支配后6周，初级突触裂隙变窄并变平。次级突触裂隙仍然保留。去神经后9周，在FJS观察到类似自主神经的神经末梢。其中一些终端W ...更多信息用5-OHDA标记。此外，血管和许旺氏管周围的自主神经增多。这些结果表明，失神经支配的新生神经纤维是肾上腺素能神经，自主神经延伸到FJS形成突触接触，虽然失神经支配后FJS的超微结构发生了显著变化，但AchE活性在很长一段时间内相对稳定。为了检查神经支配的喉肌中的NMJ，在切断左侧RLN后立即进行神经的“端对端”缝合。缝合后两周，FJS处尚未观察到神经末梢。三周后，在三分之一的FJS中发现了新形成的神经末梢，证实运动神经纤维的神经递质是Ach。电镜下可见CGRP免疫反应阳性神经末梢。这些结果表明，CGRP参与调节喉内肌的复杂收缩功能。少