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Immunohistochemical and ultrastructural study of salivary gland diseases causing disturbance of salivary secretion

引起唾液分泌紊乱的唾液腺疾病的免疫组织化学和超微结构研究

基本信息

批准号：
08671986
负责人：
YOSHIHARA Toshio
金额：
$ 1.41万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Salivary gland tissues in patients of Sjogren's syndrome and sialadenosis and in aged subjects were investigated histopathologically. We also studied the characteristics of the secretory granules in acinic cell carcinoma (acc), the normal adult and fetal parotid glands. 1. Parotid gland tissues in Sjogren's syndrome were characterized by atrophy of acini, lymphocyte infiltration and the presence of epimyoepithelial islands. The three-dimensional reconstructive study showed that epimycepithelial islands were not mere cell clusters, and derived from ductal cells rather than myoepithelial cells. Mutual correlation between aging and residual rate of acini was observed in both female and male subjects. Female subjects showed a higher coefficient of correlation. 2. The acinar cells of the parotid gland in sialadenosis were pale and swollen. Ultrastructurally, most secretory granules were homogeneous and electron lucent, and they were positive for PAM, amylase and several lectins. The present results indicated that the protein (amylase) synthesis existed in the acinar cells in patients of sialadenosis, but the formation of cores in granules which stored amylase was disturbed. 3. Ultrastructural characteristics of the secretory granules of acc was similar with those of fetal parotid glands rather the adult parotid acinar cells. PAM staining and lectin cytochemistry were used in this study. The ultrastructural and cytochemical results showed that the secretory granules in acc resembled those of fetal parotid glands. It is suggested that the tumor cells of acc form immature secretory granules of the fetal type as a result of undifferentiation of the gland cells.

本文对干燥综合征、涎腺腺病和老年人的涎腺组织进行了组织病理学观察。我们还研究了腺泡细胞癌（acc）、正常成人和胎儿腮腺分泌颗粒的特征。1.干燥综合征的腮腺组织以腺泡萎缩、淋巴细胞浸润和肌上皮岛的存在为特征。三维重建的研究表明，epimycepithelial岛不仅仅是细胞簇，并来自导管细胞，而不是肌上皮细胞。男性和女性的年龄与腺泡残留率存在相关性。女性受试者表现出较高的相关系数。2.腮腺腺泡细胞苍白、肿胀。电镜下，大部分分泌颗粒均质，电子透明，PAM、淀粉酶和多种凝集素阳性。结果表明，涎腺腺泡细胞中存在蛋白质（淀粉酶）合成，但储存淀粉酶的颗粒核心的形成受到干扰。3.腺样囊性癌分泌颗粒的超微结构特征与胎儿腮腺腺泡细胞相似，而与成人腮腺腺泡细胞相似。本研究采用PAM染色和凝集素细胞化学方法。超微结构和细胞化学结果表明，acc中的分泌颗粒与胎儿腮腺的分泌颗粒相似。提示acc的肿瘤细胞由于腺细胞未分化而形成胎儿型的未成熟分泌颗粒。