A development of antiplaque composite resin by chemically binding Dextranase

化学结合葡聚糖酶抗牙菌斑复合树脂的研制

• 批准号: 02670851
• 负责人: YASUNAGA Tetsuya
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670851/
• 关键词: Dextranase; Antiplaque Activity; Immobilized Enzyme; Water Insoluble Glucan

The experimental resin was prepared : Bis-GMA 55.3%, TEGDMA 27.6%, polyacrylic acid 16.6%, BPO 0.5%. This resin was polymerized for 24 h at 60ﾟC and 4 h at 100ﾟC. The discs of this resin(10mm in diameter and 1mm thickness)were prepared and stored in 0.1M-potassium phosphate buffer solution(pH6.0). Dextranase was bonded to the disc by the following procedure. One disc and N-ethyl-5-phenylisoxazolium3'-sulfonate(40mg)were stirred for 1 h at 4ﾟC in 10ml of 0.1M-potassium phosphate buffer(pH6.0). Dextranase(40units)were then added, and the reaction mixture stirred for 24 h at 4ﾟC. 0.11 units of dextranase activity was found on a disc.The effect of dextranase bound resin on the glucosyltransferase of Streptococcus mutans was investigated. Specimens of seven materials were prepared : 1. the dextranase bound experimental resin, the untreated experimental resin, Unifast, Fuji Ionomer TypeII, Photoclearfil Bright, Silux Plus, Palfique Estelite. Water insoluble glucan synthesizd by glucosyltransferase on the specimens were measured. The dextranase bound resin specimen inhibited water insoluble glucan synthesis more strongly than the another specimens. The percent inhibition of water insoluble glucan formation by glucosyltransferase on the dextranase bound resin specimen was about 30% of the untreated resin specimen.We thus examined the adherent ability of Streptococcus mutans MT6R and glucan production by this organism on the dextranase bound experimental resin. Scaning electron microscope was utilized to make this examination. The dextranase bound experimental resin inhibited the adherence of Streptococcus mutans more strongly than the untreated experimental resin.From this investigation it was concluded that this dextranase bound experimental resin have antiplaque activity.

实验树脂的配方为：Bis-GMA 55.3%，TEGDMA 27.6%，聚丙烯酸16.6%，BPO 0.5%。该树脂在60 ℃下聚合24小时，在100 ℃下聚合4小时。制备直径10 mm、厚1 mm的树脂片，置于0.1M磷酸钾缓冲液（pH6.0）中保存。通过以下程序将葡聚糖酶结合到椎间盘上。将一个圆盘和N-乙基-5-苯基异恶唑鎓 3 ′-磺酸盐（40 mg）在10 ml 0.1M磷酸钾缓冲液（pH6.0）中于4 ℃搅拌1小时。然后加入右旋糖酐酶（40单位），并将反应混合物在4 ℃下搅拌24小时。0.11本文研究了葡聚糖酶结合树脂对变形链球菌葡萄糖基转移酶的影响。制备了七种材料的标本：1。葡聚糖酶结合的实验树脂、未处理的实验树脂、Unifast、Fuji Iceland Type II、Photoclearfil Bright、Silux Plus、Palfique Estelite。测定了样品上由葡糖基转移酶合成的水不溶性葡聚糖。葡聚糖酶结合的树脂样品比其他样品更强烈地抑制水不溶性葡聚糖的合成。葡糖基转移酶对葡聚糖酶结合的树脂样品上的水不溶性葡聚糖形成的抑制百分比约为未处理的树脂样品的30%。因此，我们检测了变形链球菌MT 6 R的粘附能力和该生物体在葡聚糖酶结合的实验树脂上的葡聚糖生产。用扫描电子显微镜进行观察。葡聚糖酶结合的实验树脂对变形链球菌的粘附抑制作用强于未处理的实验树脂，表明葡聚糖酶结合的实验树脂具有抗菌斑活性。