Reseach for augmentation of Interleukin-2 activated killer activity

我们应用LAK细胞进行过继免疫治疗。为有效地进行过继免疫治疗，我们进行了三项研究：研究一：从鳞癌患者外周血淋巴细胞和正常人外周血淋巴细胞中诱导LAK细胞，比较两种LAK细胞培养后的细胞毒活性和淋巴细胞亚群。结果表明，人外周血LAK细胞对K562和Daudi的杀伤活性与正常人相当。PWM和PHA均能增强K562细胞的细胞毒活性。研究3：我们尝试了cFDA和流式细胞术检测细胞毒活性的新方法。该方法与铬释放测定法具有良好的相关性。

We have applied adoptive immunotherapy by LAK cells. We maked three studies for the purpose of using effectively adoptive immunotherapy.Study 1 : LAK cells induced from PBL patierits with scqueamous carcinoma and of normal human, cytotoxic activity and lymphocyte subsets after culture were compared two kinds of LAK cells. In LAK cells from PBL of scqueamous carcinoma patients, cytotoxic activity against K562 and Daudi was as high as activity from normal human.Study 2 : We tried to augment cytotoxic activity by using PWM or PHA after induction of LAK cells. Augmentaion of cytotoxic activity against K562 was shown by using both PWM and PHA. But augmentaion of cytotoxic activity against Daudi was shown by using PHA only.Study 3 : We tried new method for assay of cytotoxic activity by using cFDA and flowcytometry. This new method showed correlation to Cr release assay.