特異的抗腫瘍効果を期待した放射線療法との併用による新しい養子免疫療法の確立

本研究的目的是通过放射治疗增加靶细胞上MHC Ⅰ类抗原的表达来增强过继免疫治疗的效果（IFN-γ）.2.巨噬细胞因子激活的杀伤细胞（LAK）的变化人鳞状细胞癌细胞系对MHC Ⅰ类抗原敏感性的研究3.放射线和/或IFN-γ引起的鳞状细胞癌细胞系NRS 1上MHC Ⅰ类抗原的增加4. LAK细胞对NRS 1上MHC Ⅰ类抗原敏感性的变化（C57BL/6，Balb/c）LAK细胞随着NRS 1上MHC Ⅰ类抗原的增加而减少。5. NRS 1上MHC Ⅰ类抗原的清除作用经pH3.0试剂处理后消失。6. LAK细胞随着NRS 1上MHC Ⅰ类抗原的清除作用的变化抗原对小鼠同种异体抗原的细胞毒敏感性（C57BL/6，Balb/c）LAK细胞随着NRS Ⅰ类抗原的消除而增加。7.参与LAK细胞敏感性变化的LAK细胞亚群的研究本研究表明，GM 1抗原阳性细胞参与了LAK细胞对NRS 1的MHC Ⅰ类抗原依赖性的敏感性改变，并进一步阐明了GM 1抗原阳性细胞在LAK细胞杀伤活性改变中的作用。CD 8抗原阳性细胞对LAK细胞敏感性的改变。

The purpose of this study is buildup adoptive immunotherapy effect with increase of MHC class I antigen on target cells by radiation.1.Increase of MHC class I Antigen on Four Kinds of Cell Lines of Human Squamous Cell CartinomaMHC class I antigen on cell lines of human squamous cell cartinoma increase by radiation and/or interferon-gamma (IFN-gamma).2.Changes of Lymphokine Activated Killer (LAK) Sensitivity with Increase of MHC class I Antigen on Cell Lines of Human Squamous Cell CartinomaThe sensitivity of cytotoxicity against human allogenic LAKcells is decreased with increase of MHC class I antigen.3.Increase of MHC class I Antigen on Murine Cell Line of Squamous Cell Cartinoma NRS1MHC class I antigen on NRS1 increased by radiation and/or IFN-gamma.4.Changes of LAK Sensitivity with Increase of MHC class I Antigen on NRS1The sensitivity of cytotoxicity against murine syngenic (C3H/He) and allogenic (C57BL/6, Balb/c) LAK cells is decreased with increase of MHC class I antigen on NRS1.5.Elimination of MHC class I Antigen on NRS1MHC class I antigen on NRS1 disappeared by treatment of pH3.0 reagent.6.Changes of LAK Sensitivity with elimination of MHC class I antigenThe sensitivity of cytotoxicity against murine allogenic (C57BL/6, Balb/c) LAK cells is increased with elimination of MHC class I antigen on NRS1.7.Investigation for Subpopulation in LAK Cells which take part in the Changes of LAK SensitivityIt suggest that asialo-GM1 antigen positive cells participate in the change of LAK sensitivity dependent on MHC class I antigen of NRS1 in this study.we are investigatingthe role of of CD8 antigen positive cells in the change of LAK sensitivity.