特異的抗腫瘍効果を期待した放射線療法との併用による新しい養子免疫療法の確立

建立新的过继性免疫疗法联合放疗，有望产生特异性抗肿瘤效果

• 批准号: 04671246
• 负责人: KOSE Akira
• 金额: $ 1.41万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671246/
• 关键词: Radiation; IFN-gamma; LAK cell; MHC class I antigen; Oral squeamous cartinoma; Immunotherapy; LAK感受性; インターフェロンgamma; 養子免疫療法; 放射線療法; LAK; MHC抗原

The purpose of this study is buildup adoptive immunotherapy effect with increase of MHC class I antigen on target cells by radiation.1.Increase of MHC class I Antigen on Four Kinds of Cell Lines of Human Squamous Cell CartinomaMHC class I antigen on cell lines of human squamous cell cartinoma increase by radiation and/or interferon-gamma (IFN-gamma).2.Changes of Lymphokine Activated Killer (LAK) Sensitivity with Increase of MHC class I Antigen on Cell Lines of Human Squamous Cell CartinomaThe sensitivity of cytotoxicity against human allogenic LAKcells is decreased with increase of MHC class I antigen.3.Increase of MHC class I Antigen on Murine Cell Line of Squamous Cell Cartinoma NRS1MHC class I antigen on NRS1 increased by radiation and/or IFN-gamma.4.Changes of LAK Sensitivity with Increase of MHC class I Antigen on NRS1The sensitivity of cytotoxicity against murine syngenic (C3H/He) and allogenic (C57BL/6, Balb/c) LAK cells is decreased with increase of MHC class I antigen on NRS1.5.Elimination of MHC class I Antigen on NRS1MHC class I antigen on NRS1 disappeared by treatment of pH3.0 reagent.6.Changes of LAK Sensitivity with elimination of MHC class I antigenThe sensitivity of cytotoxicity against murine allogenic (C57BL/6, Balb/c) LAK cells is increased with elimination of MHC class I antigen on NRS1.7.Investigation for Subpopulation in LAK Cells which take part in the Changes of LAK SensitivityIt suggest that asialo-GM1 antigen positive cells participate in the change of LAK sensitivity dependent on MHC class I antigen of NRS1 in this study.we are investigatingthe role of of CD8 antigen positive cells in the change of LAK sensitivity.

本研究的目的是通过辐射增强MHC-I类抗原对靶细胞的过继免疫治疗作用。1.人鳞状细胞癌四种细胞系MHC-I类抗原的增加；3.人鳞状细胞癌细胞系MHC-I类抗原的增加；2.人鳞状细胞癌细胞系LAK敏感性随MHC-I类抗原的增加而变化小鼠鳞状细胞癌NRS1MHC-I类抗原对NRS1的敏感性随NRS1-MHC-I类抗原的增加而增加。BALB/c)LAK细胞随NRS1.5上MHC-I类抗原的增加而减少。NRS1上MHC-I类抗原的清除在pH3.0试剂处理下消失。6.LAK敏感性随MHC-I类抗原的消除而变化。LAK细胞中参与LAK敏感性改变的亚群的研究表明，在本研究中，依赖于NRS1的MHC-I类抗原，asialo-GM1抗原阳性细胞参与了LAK敏感性的改变，并探讨CD8抗原阳性细胞在LAK敏感性改变中的作用。