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Effects of LAK cell on Growth and Metastasis of the Murine B16-F10 Melanoma

LAK细胞对小鼠B16-F10黑色素瘤生长和转移的影响

基本信息

批准号：
61570485
负责人：
UNO Akihiko
金额：
$ 0.64万
依托单位：
Yamanashi Medical College (1987)山梨医科大学 (1986)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570485/
关键词：
rIL-2 LAK細胞 LAK活性 IL-【II】

项目摘要

To study the effect of lymphokine-activated killer cells (LAK) in vivo, we investigated the suppression of the primary tumor growth and the inhibition of metastasis to the lunge in C57BL/5 mice by adoptive transfer of LAK cells. In this expriment, we used B16-F10 murine melanoma cells which were selected in vivo for high pulmanary colonization.1. Fastly we investigated the effect of LAK cells in vivo to the suppressions of the primary tumor growth. 5x10^6 melanoma cells were inoculated into the outer ear of C57BL/6 mice. After 10 days, LAK cells were in transfered on 5 days. 30 days later, tumor weight was checked for LAK cell treated group and non treated group, primary tumor in mice weighted 40-50% less than those in control mice. LAK cells transfusion was effective on the suppression of tumor growth.2. We tried to examine the effect of LAK cells in vivo on the pulmanary colonizations of C57BL/6 mice after intravenous ingection of 5x10^4 melanoma cells. Fathermor, r-IL2 (1x10^3U/ml) was applied by intra-peritoneal injection. The metastatic property was determined by counting the lung colonies 20 day after intravenous injection. The average number of pulmanary colonization in control group were 68<plus-minus>20, in a combination (LAK cell and r-IL2) transfer group were 32<plus-minus>18 respectively. This result showed that transfer of LAK cells were effective on the inhibition of matastasis. Fathermore, the effect of inhibition were enchanced by treatment of rIL-2.

为研究LAK细胞在体内的作用，我们采用过继转移的方法，观察了LAK细胞对C57 BL/5小鼠原发性肿瘤生长的抑制作用和对肺癌转移的抑制作用。在本实验中，我们使用了B16-F10小鼠黑色素瘤细胞，这些细胞在体内被选择用于高肺定殖。本文首先研究了LAK细胞在体内对原发性肿瘤生长的抑制作用。将5x10^6个黑素瘤细胞接种到C57 BL/6小鼠的外耳中。10天后，第5天转移LAK细胞。30天后，检测LAK细胞治疗组和未治疗组小鼠的肿瘤重量，原发性肿瘤重量比对照组轻40-50%。LAK细胞输注对肿瘤生长有抑制作用.我们试图检测静脉注射5 × 10^4黑色素瘤细胞后，体内LAK细胞对C57 BL/6小鼠肺内定殖的影响。通过腹膜内注射施用Fathermor，r-IL 2（1x10^3U/ml）。通过静脉注射后20天计数肺集落来确定转移特性。对照组肺内平均定植数为68.20个<plus-minus>，LAK细胞与r-IL-2联合移植组肺内平均定植数为<plus-minus>32.18个。结果表明，LAK细胞移植对肿瘤转移有明显的抑制作用。rIL-2处理后，抑制作用增强。