Effects of LAK cell on Growth and Metastasis of the Murine B16-F10 Melanoma

为了研究淋巴因子激活杀伤细胞（LAK）在体内的作用，我们研究了LAK细胞过继转移对C57BL/5小鼠原发肿瘤生长的抑制作用和对肺转移的抑制作用。在本实验中，我们使用在体内选择的B16-F10小鼠黑色素瘤细胞进行高肺定植。我们在体内研究了LAK细胞对原发肿瘤生长的抑制作用。将5x10^6黑色素瘤细胞接种于C57BL/6小鼠外耳。10 d后，LAK细胞在第5天转移。30 d后，检测LAK细胞处理组和未处理组的肿瘤重量，小鼠原发肿瘤重量比对照组轻40-50%。LAK细胞输注对肿瘤生长有明显的抑制作用。我们试图检测静脉注射5x10^4黑色素瘤细胞后LAK细胞在体内对C57BL/6小鼠肺部定植的影响。父亲莫尔，r-IL2 （1 × 10^3U/ml）腹腔注射。通过静脉注射后20天的肺菌落计数来确定转移性。对照组平均定植68<正负>20，LAK细胞与r-IL2联合转染组平均定植32<正负>18。结果表明，LAK细胞的转移对肿瘤转移有明显的抑制作用。此外，il -2的处理增强了抑制作用。

To study the effect of lymphokine-activated killer cells (LAK) in vivo, we investigated the suppression of the primary tumor growth and the inhibition of metastasis to the lunge in C57BL/5 mice by adoptive transfer of LAK cells. In this expriment, we used B16-F10 murine melanoma cells which were selected in vivo for high pulmanary colonization.1. Fastly we investigated the effect of LAK cells in vivo to the suppressions of the primary tumor growth. 5x10^6 melanoma cells were inoculated into the outer ear of C57BL/6 mice. After 10 days, LAK cells were in transfered on 5 days. 30 days later, tumor weight was checked for LAK cell treated group and non treated group, primary tumor in mice weighted 40-50% less than those in control mice. LAK cells transfusion was effective on the suppression of tumor growth.2. We tried to examine the effect of LAK cells in vivo on the pulmanary colonizations of C57BL/6 mice after intravenous ingection of 5x10^4 melanoma cells. Fathermor, r-IL2 (1x10^3U/ml) was applied by intra-peritoneal injection. The metastatic property was determined by counting the lung colonies 20 day after intravenous injection. The average number of pulmanary colonization in control group were 68<plus-minus>20, in a combination (LAK cell and r-IL2) transfer group were 32<plus-minus>18 respectively. This result showed that transfer of LAK cells were effective on the inhibition of matastasis. Fathermore, the effect of inhibition were enchanced by treatment of rIL-2.