Analysis of endocrine-metabolic systems during exercise and clinical applications of exercise for laboratory test.

运动过程中内分泌代谢系统分析以及运动实验室测试的临床应用。

• 批准号: 02671067
• 负责人: HASHIMOTO Takuma
• 金额: $ 1.34万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671067/
• 关键词: Exercise testing; Hormones; Endocrinology; Chemical substances; physiological Test; hypothalamo-pitinitary function test; Laboratory medicine; Ergometer; 内分泌機能検査; 下垂体機能検査; 甲状腺機能検査; 甲状腺ホルモン代謝; 内分泌学; 臨床検査医学; 自転車エルゴメ-タ-

Exercise can affect numerous biochemical variables. We consider it is very important to clarify the effect of exercise(short-term exercise e. g. a bicycle ergometer at 60% VO_2max for 15 min.) in order to establish a reliable physiological loading test in the clinical laboratory fields. We therefore decided to investigate I)the effect exercise on endocrinological organs 2)the effect of exercise on the blood chemical variables(laboratory tests)3)the simultaneous effect of the hormonal changes on the blood chemistry and the relationship between them. One hundred and thirty male students, aged 23-26 yr, were subjected to exercise on a bicycle ergometer at 60% VO_2max for 15 min. Blood was collected just before and immediately after exercise. Plasma catecholamines(adrenaline(AD), noradrenaline(NAD)and doparmine)were measured using the HPLC method. Other serum hormones were measured by RIA or EIA and 70 serum chemicals were determined by routine methods. After exercise, mean AD, NAD, ACTH, … More PRL, GH, TSH, Aldosterone, renin, cortisol, gulcagon, FT_3, FT_4, PTH, osteocalcin, ADH, HANP, somatomedine C, Vitamine D_3, erythropoietin, and thyroxine binding globulin(TBG)showed a significant increase. On the other hand, serum calcitonin, insulin and c-peptide decreased signifidantly. Dopamine, LH, FSH, estradiol, somatostatin, motilin, VIP, CCK, secretin, substance P and neurotensin showed no change. Thirty two out of 70 chemical substances(enzymes, lipids and electrolytes etc.)were significantly increased but 4 decreased. The greatest increase was in NEFA(non-estrified free fatty acid)and the greatest decrease was in chylomicrons. The relationship of % changes of all determinants was calculated using a computer. Many correlations between hormones and biochemical components were found. For example, we revealed 24 correlations between thyroid and parathyroid hormones and biochemical components. We firstly demonstrated the importance of FT3 not only in protein metabolism but also in lipid metabolism. Further studies on exercise-induced hormonal change and the interrelationship between hormones and biochemical variables should be carried out. Less

运动可以影响许多生化变量。我们认为，为了在临床实验室领域建立可靠的生理负荷测试，阐明运动（短期运动，例如在60% VO_2max下骑自行车测力器15分钟）的效果是非常重要的。因此，我们决定研究1)运动对内分泌器官的影响2)运动对血液化学变量的影响（实验室测试）3)激素变化对血液化学的同时影响及其之间的关系。130名年龄在23-26岁的男学生在自行车测力仪上以60%最大摄氧量运动15分钟。在运动前和运动后立即采集血液。采用高效液相色谱法测定血浆儿茶酚胺（肾上腺素（AD）、去甲肾上腺素（NAD）和多巴胺）。其他血清激素采用RIA或EIA法测定，70种血清化学物质采用常规方法测定。运动后AD、NAD、ACTH、PRL、GH、TSH、醛固酮、肾素、皮质醇、谷胱甘肽、FT_3、FT_4、PTH、骨钙素、ADH、HANP、生长激素C、维生素D_3、促红细胞生成素、甲状腺素结合球蛋白（TBG）均显著升高。血清降钙素、胰岛素、c肽均显著降低。多巴胺、LH、FSH、雌二醇、生长抑素、运动素、VIP、CCK、分泌素、P物质、神经紧张素无变化。70种化学物质中有32种（酶、脂类、电解质等）显著升高，4种显著降低。非甾体游离脂肪酸（NEFA）增加最多，乳糜微粒减少最多。用计算机计算了各决定因素百分比变化的关系。发现了激素与生物化学成分之间的许多相关性。例如，我们揭示了甲状腺和甲状旁腺激素与生化成分之间的24种相关性。我们首先证明了FT3不仅在蛋白质代谢中重要，而且在脂质代谢中也很重要。运动引起的激素变化及其与生化变量的相互关系有待进一步研究。少

项目成果

橋本 琢磨,他: "下垂体ー甲状腺ホルモン測定法における最近の進歩 ーnon RI法の普及と評価ー" 臨床病理. 39:9. 913-919 (1991)

Takuma Hashimoto 等人：“垂体和甲状腺激素测量方法的最新进展 - 非 RI 方法的普及和评估”临床病理学 39：919（1991）。

Takuma Hashimoto et al.: "The Importance of primary quality control ; Improving detection of low thyroxine-binding globulin levels using commercial TBG radioimmunoassay kits." JJCLA. 16. 29-33 (1991)

Takuma Hashimoto 等人：“初级质量控制的重要性；使用商业 TBG 放射免疫测定试剂盒改进低甲状腺素结合球蛋白水平的检测。”

Takuma Hashimoto et al.: "The effect of exercise on pituitary-thyroid and parathyroid function." Proceedings of the 5th Asian-Pacific Congress of Clinical Biochemistry.Excerpta Medica.

Takuma Hashimoto 等人：“运动对垂体甲状腺和甲状旁腺功能的影响。”

Takuma Hashimoto et al.: "The effect of exercise on the results of clinical laboratory tests." Proceedings of the 5th Asian-Pacific Congress of Clinical Biochemistry.Excerpta Medica.

Takuma Hashimoto 等人：“运动对临床实验室测试结果的影响。”

Takuma Hashimoto: "The effects of exercise on pituitaryーthyroid and parathyroid function in normal subjects." Endocrinol Jpn. (1991)

Takuma Hashimoto：“运动对正常人垂体甲状腺和甲状旁腺功能的影响。”Endocrinol Jpn。