Development of novel marker loci of rat by detecting variable number of tandem repeats using polymerase chain reaction.

通过使用聚合酶链式反应检测可变数量的串联重复来开发大鼠的新标记位点。

基本信息

  • 批准号:
    02680043
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1991
  • 项目状态:
    已结题

项目摘要

Identification of genetic markers exhibiting variations among inbred rat s&W are highly desired. In the present study, some marker loci were established by detecting variable number of tandem repeats(VNTR)using polymerase chain reaction(PCR).A polymorphism of VNTR in rat interleukin-3 gene, Il-3, was investigated by PCR. There were detected four types of the amplified fragments in 28 inbred rat strains. Searegation analysis revealed the linkage between Il-3 locus and Gh, RatNGFRR, or RatABPG loci, which are located on rat chromosome 10. It was thus concluded that the Il-3 locus is located on rat chromosome 10 with following order of loci ; Gh-RatNGFRR-RatABPG-Il-3.The rat seminal vesicle secretion(SVS)IV gene also contains a VNTR. This region was amplified using PCR and tnree alleles were found in 24 strains. This variation segregated as an autosomal codominant manner. We designated this locus as Svs-4. Analysis of linkages between the Svs-4 locus and other loci revealed that this locus is closely linked to Svp-1 and a loci, which belong to rat linkage group IV. Therefor the Svs-4 loci is revealed to belong to the linkage group IV.Microsatellite sequences of rat containing dinucleotide repeats were searched in DNA databases. Among the obtained microsatellites sequences, several sequences were analyzed by PCR to examine size variation in inbred rat strains. All of the microsatellite sequences were variable in size, indicating that the microsatellite loci should be useful marker for linkage analyses of the rat.It was thus concluded that the VNTR loci should be powerful tool for constructing linkage map of the rats. We are now progress in constructing the maps of particular chromosomes of the rats by linkage analyses using the VNTR loci.
在近交系大鼠s&W中表现出变异的遗传标记的鉴定是高度期望的。本研究采用聚合酶链反应(PCR)技术检测大鼠IL-3基因可变数目串联重复序列(VNTR),建立了VNTR的标记位点,并对IL-3基因VNTR的多态性进行了研究。在28个品系的近交系大鼠中检测到4种类型的扩增片段。基因定位分析表明IL-3基因座与位于大鼠10号染色体上的Gh、RatNGFRR和RatABPG基因座连锁。结论:IL-3基因位于大鼠第10号染色体上,其顺序为:Gh-RatNGFRR-RatABPG-IL-3。大鼠精囊分泌(SVS)IV基因也含有一个VNTR。用PCR扩增该区域,在24株菌株中发现3个等位基因。这种变异以常染色体共显性方式分离。我们将这个位点命名为Svs-4。Svs-4位点与其他位点之间的连锁分析表明,该位点与Svp-1和a位点紧密连锁,属于大鼠连锁群IV。因此,Svs-4位点属于连锁群IV。在DNA数据库中检索了含有二核苷酸重复序列的大鼠微卫星序列。在获得的微卫星序列中,通过PCR分析了几个序列,以检查近交系大鼠品系的大小变化。所有微卫星序列大小可变,表明微卫星位点可作为大鼠遗传连锁分析的有效标记,VNTR位点可作为构建大鼠遗传连锁图谱的有力工具。利用VNTR基因座进行连锁分析,构建大鼠特定染色体图谱的工作正在取得进展。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kunieda,T.et al.: "Polymorphisms detected in actin-related sequences of rat (RaHus norvegicus)" Experimental Animals. 39. 307-310 (1990)
Kunieda,T.et al.:“在大鼠 (RaHusnorvegicus) 肌动蛋白相关序列中检测到的多态性”实验动物。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
kunieda, T., Kobayashi, E., Tachibana, M., Ikadai, H., and Imamichi T.: "Polymorphic microsatellite loci of rat (Rattus norvegicus) analyzed by polymerase chain reaction." Genomics.
kunieda, T.、Kobayashi, E.、Tachibana, M.、Ikadai, H. 和 Imamichi T.:“通过聚合酶链式反应分析大鼠(褐家鼠)的多态性微卫星位点。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Kunieda,T.et al.: "Localization of interleakin-3 gene to rat chromosome 10 by linkage analyses using variable number of tandem repeats(VNTR)" Mammalian Genomes.
Kunieda,T.et al.:“通过使用可变串联重复次数 (VNTR) 的连锁分析将 interleakin-3 基因定位到大鼠 10 号染色体”哺乳动物基因组。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Eiji Kobayashi 他: "Genetic profiles of II inbred rat strains at 25 biochemical marker loci and five RFLD loci." Laboratory Animal Science. 4. 1-3 (1992)
Eiji Kobayashi 等人:“II 近交大鼠品系的 25 个生化标记基因座和 5 个 RFLD 基因座的遗传概况。实验动物科学”4. 1-3 (1992)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
T.Knnieda,et al: "DNA polymorphism detected in minisalellite region of rat ILー3 gene" Immunogenetics.
T. Knnieda 等人:“在大鼠 IL-3 基因的小卫星区域中检测到的 DNA 多态性”免疫遗传学。
  • DOI:
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  • 影响因子:
    0
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KUNIEDA Tetsuo其他文献

KUNIEDA Tetsuo的其他文献

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{{ truncateString('KUNIEDA Tetsuo', 18)}}的其他基金

Investigation for effects of blood coagulation factor XI deficiency on productivity to the genetic improvement of Japanese beef cattle
凝血因子XI缺乏对日本肉牛遗传改良生产力影响的研究
  • 批准号:
    23380166
  • 财政年份:
    2011
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Genetic investigation of the genes responsible for hereditary disorders of Japanese Black cattle and its functional analysis
日本黑牛遗传性疾病基因的遗传学调查及其功能分析
  • 批准号:
    20248029
  • 财政年份:
    2008
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Molecular cloning and functional analysis of gene responsible for multiple ocular defect of cattle
牛多发性眼部缺损基因的分子克隆及功能分析
  • 批准号:
    18380167
  • 财政年份:
    2006
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
MORECULAR GENETICAL INVESTIGATION AND DNA DIAGNOSTIC SYSTEM FOR BOVINE CHONDRODYSPLASTIC DWARFISM
牛软骨发育不良性侏儒症的更常规遗传学调查和 DNA 诊断系统
  • 批准号:
    11556055
  • 财政年份:
    1999
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
MORECULAR GENETICAL INVESTIGATION OF DOMESTIC ANIMALS AND POULTRY USING HIGHLY POLYMORPHIC MICROSATELLITE DNA
使用高度多态性微卫星 DNA 对家畜和家禽进行更规则的遗传研究
  • 批准号:
    08456152
  • 财政年份:
    1996
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
ESTABLISHMENT OF A RAT STRATIN WITH MUCOPOLYSUCCHARIDOSIS TYPE VI AS AN ANIMAL MODEL FOR GENE THERAPY
粘多糖症VI型大鼠模型的建立作为基因治疗的动物模型
  • 批准号:
    07558240
  • 财政年份:
    1995
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)

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