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The role of Harderian gland derived growth factor and growth inhibitor in the development of Harderian gland

哈氏腺衍生生长因子和生长抑制剂在哈氏腺发育中的作用

基本信息

批准号：
02680127
负责人：
KANO Kazutaka
金额：
$ 1.54万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The Harderian gland is well developed in rodents and in some other animals. The guinea pig Harderian gland secretes 1-alkyl- 2,3-diacylglycerol that may facilitate the movement of the eye. Although several actions other than lubrication have been reported, the precise role of the Harderian gland remain to be fully elucidated. We have purified a growth factor from the Harderian gland of guinea pig and named it Harderian gland derived growth factor (HGDGF). This factor differs from PDGF, TGF, IGF and EGF, but resembles FGF. However, this factor did not stimulate human endothelial cells which require fibroblast growth factor. These results show that HGDGF differs from FGF and is a previously undescribed factor. We also found a growth inhibitory factor in the guinea pig Harderian gland which inhibited the growth of cultured Harderian gland cells. In this study, I intended to elucidate the physiological role of the growth stimulating or growth inhibiting factors in the development and the maintenance of the Harderian gland and surrounding tissues.A part of cDNA has been cloned and isolation of full size mRNA from cultured Harderian gland cells is now in progress. The HGDGF stimulated the growth of cultured cornea stromal cells. The combined use of HGDGF with other growth factor such as FGF and PDGF increased the cell growth. The growth stimulating activity of HGDGF was inhibited by suramin in a different manner from that of FGFs. These findings suggest that HGDGF plays a role in the repair of injured parts of the cornea and in the maintenance of the cornea stromal cells. I also established the cultured guinea pig harderian gland cells maintaining the lipid synthesis. The cultured cell system will be useful tool for the analysis of the role of growth factors in the Harderian gland.

哈德氏腺在啮齿动物和其他一些动物中发育良好。豚鼠哈德氏腺分泌1-烷基-2，3-二酰基甘油，这可能有助于眼睛的运动。虽然已经报道了除润滑之外的其他几种作用，但哈德氏腺的确切作用仍未完全阐明。我们从豚鼠Harderian腺体中分离纯化出一种生长因子，命名为Harderian腺源性生长因子(HGDGF)。该因子不同于PDGF、转化生长因子、IGF和EGF，但与成纤维细胞生长因子相似。然而，该因子不能刺激需要成纤维细胞生长因子的人内皮细胞。这些结果表明，HGDGF不同于成纤维细胞生长因子，是一个以前未被描述的因素。我们还在豚鼠哈德氏腺中发现了一种生长抑制因子，它能抑制培养的哈德氏腺细胞的生长。本研究旨在阐明生长促进因子或生长抑制因子在哈德氏腺及其周围组织的发育和维持中的生理作用。目前已克隆了部分cDNAs，并正在从培养的哈德氏腺细胞中分离全长的mRNA。HGDGF对培养的角膜基质细胞有促进生长的作用。HGDGF与其他生长因子如成纤维细胞生长因子、血小板衍生生长因子联合使用可促进细胞生长。苏拉明对HGDGF的促生长活性的抑制作用与FGFs不同。这些发现表明，HGDGF在角膜损伤部分的修复和角膜基质细胞的维持中发挥作用。我还建立了培养的豚鼠哈德氏腺细胞，维持脂质合成。培养的细胞系统将是分析生长因子在哈德氏腺中作用的有用工具。