EFFECT OF HARDERIAN GLAND DERIVED GROWTH FACTOR ON THE DEVELOPMENT OF CHICKEN EMBRYONIC NEURAL RETINA

哈氏腺源性生长因子对鸡胚神经视网膜发育的影响

• 批准号: 05680652
• 负责人: KANO Kazutaka
• 金额: $ 1.28万
• 依托单位: UNIVERSITY OF TOKYO,FACULTY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680652/
• 关键词: HGDGF; bFGF; neural retina; apoptosis; ganglion cell; 線維芽細胞成長因子; 細胞成長因子; ハーダー腺由来細胞成長因子(HGDGF); 神経細胞; ニワトリ神経網膜細胞; ラット胎児神経前駆細胞

Harderian gland derived growth factor (HGDGF) is a novel growth factor isolated from guinea pig Harderian gland. The duct of the gland opens to the third eye rids, therefore the factor is assumed to play an important role in the wound healing of the injured cornes. However, true function is remained unknown . In the previous experiments, I demonstrated the possibilities that HGDGF stimulated the proliferation and promoted the differentiation of rat brain nerve precursor cells.The object of the study is to analyze the function and the mood of action of HGDGF and other growth factor in the developing nerve systems. In the nervous systems such as chicken neural retina, neurons that fail to make correct connection or to locate their exact positions in certain circumstance die. I demonstrated that cultured day 8 chicken neural retina died by apoptosis and bFGF stimulated the process. The enhancing effect of bFGF was canceled by the addition of HGDGF or suramin. On the other hand these two factor stimulated the proliferation of rat brain nerve precursor cells in an assorted manner. other factor including TGF-alpha and beta, PDGF,NGF,aFGF,IGF-I and II,TNF did not have any effect on the process.

哈德氏腺衍生生长因子(HGDGF)是从豚鼠哈德氏腺中分离得到的一种新的生长因子。腺体的导管通向第三眼肋骨，因此该因子被认为在角膜损伤的伤口愈合中起着重要作用。然而，真正的功能仍然是未知的。在之前的实验中，我论证了HGDGF刺激大鼠脑神经前体细胞增殖和促进分化的可能性，本研究的目的是分析HGDGF和其他生长因子在神经系统发育中的作用和作用方式。在鸡的神经视网膜等神经系统中，在某些情况下无法正确连接或定位其确切位置的神经元会死亡。我证明了培养第8天的鸡神经视网膜是通过细胞凋亡而死亡的，而碱性成纤维细胞生长因子刺激了这个过程。加入HGDGF或苏拉明可拮抗bFGF的促进作用。另一方面，这两种因子以多种方式刺激大鼠脑神经前体细胞的增殖。其他因子包括转化生长因子-α和β、PDGF、NGF、aFGF、IGF-I和II、肿瘤坏死因子对此过程无影响。

项目成果

M. Maekawa, T. Iguch, K. kano: "Molewlan cloning and characterization of MMTVloci in SHN mile" J. Vicology. (in press).

M. Maekawa、T. Iguch、K. kano：“SHN 英里中 MMTVloci 的 Molewlan 克隆和表征”J. Vicology。

M. shioda, K. kano, M. Kobayashi: "Differential inhibition of eukaryotic DNA peeynrerase by halenaquinol sulfate" B. B. R. C. (in press). (1995)

M. shioda、K. kano、M. Kobayashi：“海萘醌硫酸盐对真核 DNA Peeynrerase 的差异抑制”B. B. R. C.（出版中）。

M.Maekawa, T.Watanabe, A.Takahashi, T.Ishizawa, J.Kusunoki, T.Iguchi, S.Takasugi, Y.Seyama, Y.Yokoyama and K.Kano: "Molecular cloning and characterization of MMTV loci in SHN mouse." J.Virology. (in pressd).

M.Maekawa、T.Watanabe、A.Takahashi、T.Ishizawa、J.Kusunoki、T.Iguchi、S.Takasugi、Y.Seyama、Y.Yokoyama 和 K.Kano：“SHN 中 MMTV 基因座的分子克隆和表征

K.Murofushi, K.Kaji, K.Kazutaka, M.Fukuda, M.Shioda and H.Murofushi: "Inhibition of cell proliferation by a uniqul lysophosphatidic acid, PHYLPA,isolated from Physarun polycephalum : Signaling events of a action by PHYLPA" Cell Structure and Function. 18.

K.Murofushi、K.Kaji、K.Kazutaka、M.Fukuda、M.Shioda 和 H.Murofushi：“从 Physarun polycephalum 中分离出的独特溶血磷脂酸 PHYLPA 对细胞增殖的抑制：PHYLPA 作用的信号事件”

K.Murofushi: "Inhibition of cell prolibiration by a angue lysophusphatibuc acid isoiated from physarum polyophrlum" Cell Structure and Function. 18. 363-370 (1993)

K.Murofushi：“从多泡菌中分离出的溶血溶血酸对细胞增殖的抑制”细胞结构和功能。