Differential Expression Proteomics: Identification and Quantitation of Peptides and Proteins by Fixed Charge Derivatization and Tandem Mass Spectrometry

该提案的目的是开发新的策略，用于快速，灵敏和选择性的识别和定量的蛋白质存在于复杂的混合物。 具体而言，将开发含有选定氨基酸的肽的同位素标记的固定电荷衍生物，其在串联质谱法之后朝向单个碎裂通道引导产物离子的形成。这种方法将提供比现有方法高2个数量级的增强的选择性和灵敏度，并且将允许在蛋白质水平上检查细胞从正常状态转化为疾病状态后发生的复杂细胞变化。

The aim of this proposal is to develop novel strategies for the rapid, sensitive and selective identification and quantitation of proteins present in complex mixtures. Specifically, isotopically labeled fixed charge derivatives of peptides containing selected amino acids will be developed that direct the formation of product ions following tandem mass spectrometry toward a single fragmentation channel. This approach will provide enhanced selectivity and sensitivity of up to 2 orders of magnitude over existing approaches, and will allow examination, at the protein level, of the complex cellular changes that occur following transformation of cells from a normal to a diseased state.