Differential Expression Proteomics: Identification and Quantitation of Peptides and Proteins by Fixed Charge Derivatization and Tandem Mass Spectrometry

差异表达蛋白质组学：通过固定电荷衍生化和串联质谱法鉴定和定量肽和蛋白质

• 批准号: DP0342771
• 负责人: Prof Gavin Reid
• 金额: $ 6.64万
• 依托单位: Ludwig Institute for Cancer Research Ltd
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0342771
• 关键词: Differential; Expression; Proteomics; Identification; Quantitation

The aim of this proposal is to develop novel strategies for the rapid, sensitive and selective identification and quantitation of proteins present in complex mixtures. Specifically, isotopically labeled fixed charge derivatives of peptides containing selected amino acids will be developed that direct the formation of product ions following tandem mass spectrometry toward a single fragmentation channel. This approach will provide enhanced selectivity and sensitivity of up to 2 orders of magnitude over existing approaches, and will allow examination, at the protein level, of the complex cellular changes that occur following transformation of cells from a normal to a diseased state.

该提案的目的是开发新的策略来快速、灵敏和选择性地识别和定量复杂混合物中存在的蛋白质。 具体来说，将开发含有选定氨基酸的肽的同位素标记的固定电荷衍生物，其在串联质谱分析后引导产物离子的形成朝向单个碎裂通道。该方法将提供比现有方法高出两个数量级的选择性和灵敏度，并且可以在蛋白质水平上检查细胞从正常状态转变为患病状态后发生的复杂细胞变化。