Photodissociation mass spectrometry for lipidome analysis

该项目旨在开发和应用基于生物分析质谱学的新方法和工作流程，以阐明脂体原本隐藏的结构多样性和分子复杂性。各种脂类的结构决定了其特定的生物学功能。因此，用于脂质分析的分析方法的一个主要要求是能够对可能存在于感兴趣的样品中的可能的数万个单独的分子脂质物种进行详细的结构表征。该项目将开发和优化新的紫外光解离-串联质谱学方法，这些方法将被整合到自动化脂体分析工作流程中，以实现全面的全球脂体分析，并揭示基本细胞信号过程中涉及的脂类的结构多样性。

This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden structural diversity and molecular complexity of the lipidome. The structure of individual lipids define their specific biological functions. A major requirement of analytical methods employed for lipid analysis on a lipidome-wide scale, therefore, is to enable the detailed structural characterisation of the, potentially, tens of thousands of individual molecular lipid species that may be present within a sample of interest. This project will develop and optimise novel, ultraviolet photodissociation-tandem mass spectrometry methods which will be integrated within an automated lipidome analysis workflow, to enable comprehensive global lipidome profiling and to reveal the structural diversity of lipids involved in fundamental cellular signalling processes.