Solving the Mysteries of Monotreme Chromosomes

解开单孔类染色体之谜

• 批准号: DP0343064
• 负责人: Prof Frank Gruetzner
• 金额: $ 27.24万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0343064
• 关键词: Solving; Mysteries; Monotreme; Chromosomes

The peculiar chromosomes of Australia's platypus and echidna have been debated for more than 30 years. Classical cytology cannot resolve the puzzling sex chromosome system, or to sort out the bizarre translocation chain (unique in vertebrates) and deduce how it segregates to make viable zyotes. I will microdissect individual chromosomes, and use DNA ?paints? from them (and gene probes isolated by them) to detect homologies between unpaired chromosomes at mitosis, meiosis and in sperm. I will use immunohistochemistry to clarify chromosome pairing and recombination at meiosis. This will answer some important general questions about chromosome behaviour and sex chromosome evolution.

澳大利亚鸭嘴兽和针鼠的特殊染色体已经争论了30多年。经典细胞学无法解决令人费解的性染色体系统，也无法理清奇怪的易位链(在脊椎动物中是独一无二的)，并推断它是如何分离产生可存活的受精卵的。我会显微解剖单个染色体，然后用DNA？颜料？从它们(和它们分离的基因探针)中检测有丝分裂、减数分裂和精子中未配对染色体之间的同源性。我将使用免疫组织化学来阐明减数分裂时的染色体配对和重组。这将回答关于染色体行为和性染色体进化的一些重要的一般性问题。