Solving the Mysteries of Monotreme Chromosomes

澳大利亚鸭嘴兽和针鼹的特殊染色体已经争论了30多年。经典的细胞学无法解决令人困惑的性染色体系统，也无法整理出奇怪的易位链（脊椎动物中独一无二的），并推断出它是如何分离以形成可存活的酵母菌的。我将显微解剖单个染色体，并使用DNA？颜料？从他们（和基因探针分离他们）来检测在有丝分裂，减数分裂和精子中未配对染色体之间的同源性。我将用免疫组织化学来阐明减数分裂时染色体的配对和重组。这将回答一些关于染色体行为和性染色体进化的重要问题。

The peculiar chromosomes of Australia's platypus and echidna have been debated for more than 30 years. Classical cytology cannot resolve the puzzling sex chromosome system, or to sort out the bizarre translocation chain (unique in vertebrates) and deduce how it segregates to make viable zyotes. I will microdissect individual chromosomes, and use DNA ?paints? from them (and gene probes isolated by them) to detect homologies between unpaired chromosomes at mitosis, meiosis and in sperm. I will use immunohistochemistry to clarify chromosome pairing and recombination at meiosis. This will answer some important general questions about chromosome behaviour and sex chromosome evolution.