Studies on the proliferation and metastasis formation of human osteosarcomas
人骨肉瘤增殖和转移形成的研究
基本信息
- 批准号:03404046
- 负责人:
- 金额:$ 18.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (A)
- 财政年份:1991
- 资助国家:日本
- 起止时间:1991 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have carried out experimental and clinical studies on the proliferation and metastasis formation of human osteosarcomas in these 4 years. Results we obtained are as follows : (1) We established three transplantable human osteosarcomas on to nude mice, and one cell line derived from human osteosarcoma in vitro. (2) Human osteosarcomas containing detectable c-myctranscripts grew more rapidly in nude mice. The level of c-fos m RNA was increased in human osteosarcomas showing high rate of spontaneous lung metastasis in nude mice. (3) Focus formations of murine osteoblastoid cell line was activated by ras-oncogene, while p53 suppressed them. (4) We established a human osteoblastoid cell line in vitro. 1alpha25 (OH) _2D_3 induced the expression of alkaline phosphatase and osteocalcin, while TGFbeta_1 reduced the expression of both osteoblastic properties. (5) We established two monoclonal antibodies (MAbs) against human osteosarcoma associated antigen which were highly specific for human osteosarcomas. Those MAbs were potentially useful for diagnosis of bone malignancy. (6) We analyzed the relationship between the prognosis of human osteosarcomas and the existence of multidrug resistance gene-1 (MDR-1). Osteosarcoma contaning MDR-1 showed apparently poor prognosis. (7) MAbs specific for human BGP and Bone sialoprotein II were produced against both proteins purified from human bones. These MAsb are expected to be a useful tool for immunohistochemical diagnosis of human osteogenic tumors. (8) We studied the expression of the member of the beta_1 integrin family and extracellular matrix ligands in osteosarcoma tissues. Primary osteosarcomas closely interacted with fibronectin through alpha_4 and possibly alpha_5 subunits of the beta_1 integrin.
我们对人骨肉瘤的增殖和转移形成进行了4年的实验和临床研究。结果如下:(1)建立了三个人骨肉瘤裸鼠移植瘤模型和一个人骨肉瘤细胞系。(2)含有可检测到的c-myctranscripts的人骨肉瘤在裸鼠中生长得更快。人骨肉瘤中c-fos mRNA表达水平增高,裸鼠自发性肺转移率高。(3)ras癌基因激活小鼠成骨样细胞系的病灶形成,而p53抑制它们。(4)我们在体外建立了人成骨样细胞系。1 α 25(OH)_2D_3诱导碱性磷酸酶和骨钙素的表达,而TGF β_1降低这两种成骨特性的表达。(5)我们建立了两株抗人骨肉瘤相关抗原的单克隆抗体,它们对人骨肉瘤具有高度特异性。这些单克隆抗体对骨恶性肿瘤的诊断有潜在的应用价值。(6)分析多药耐药基因-1(MDR-1)的存在与骨肉瘤预后的关系。MDR-1阳性的骨肉瘤预后明显差。(7)针对从人骨中纯化的两种蛋白质产生对人BGP和骨唾液蛋白II特异的MAbs。这些MAsb有望成为人类成骨肿瘤免疫组化诊断的有用工具。(8)我们研究了β_1整合素家族成员和细胞外基质配体在骨肉瘤组织中的表达。原发性骨肉瘤通过β_1整合素的α_4和可能的α_5亚基与纤连蛋白密切相互作用。
项目成果
期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Chiba Hideki, Ishii Seiichi: "A newly established cell line from normal human bone responds to 1alpha, 25-dihydroxyvitamin D_3, retinoic acid and transforming growth factor-beta_1" Tumor Research. (28). 41-50 (1993)
Chiba Hideki、Ishii Seiichi:“一种新建立的来自正常人骨的细胞系对 1α、25-二羟基维生素 D_3、视黄酸和转化生长因子-β_1 做出反应”肿瘤研究。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hideki Chiba Seiichi Ishii: "A newly established cell line from normal human bone responds to 1α,25-Dihydroxyvitamin D_3,Retinoic acid and transforming growth factor-β_1" Tumor Research. 28. 41-50 (1993)
Hideki Chiba Seiichi Ishii:“来自正常人骨的新建立的细胞系对 1α,25-二羟基维生素 D_3、视黄酸和转化生长因子 -β_1 有反应”《肿瘤研究》28. 41-50 (1993)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Toshihide Fujisaki Seiichi Ishii et al.: "In vitro chemosensitivity assay for human osteosarcoma using tumor xenografts" Clinical Orthopedics. (in press). (1994)
Toshihide Fujisaki Seiichi Ishii 等人:“使用肿瘤异种移植物对人骨肉瘤进行体外化学敏感性测定”临床骨科。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
和田 卓郎: "骨軟部腫瘍の基礎的研究の現状と将来の展望-分子生物学を中心として-" 関節外科. 12. 1245-1253 (1993)
Takuro Wada:“骨和软组织肿瘤基础研究的现状和未来展望——以分子生物学为重点”关节外科。12。1245-1253(1993)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Chiba,H.: "Establishment and characterization of a simian virus 40-immortalized osteoblastic cell line from normal human bone" Jpn.J.Cancer Res.84. 290-297 (1993)
Chiba,H.:“来自正常人骨的猿病毒 40 永生化成骨细胞系的建立和表征”Jpn.J.Cancer Res.84。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ISHII Seiichi其他文献
ISHII Seiichi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ISHII Seiichi', 18)}}的其他基金
The impact of undergraduate medical education on students choice of surgery as future career
本科医学教育对学生选择外科作为未来职业的影响
- 批准号:
24591862 - 财政年份:2012
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Influence of emotional intelligence of physicians and medical students on patient-centered care
医生和医学生情商对以患者为中心的护理的影响
- 批准号:
21590550 - 财政年份:2009
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Establishment and Analysis of the in vivo Human Tumor Angiogenesis Model
人体内肿瘤血管生成模型的建立与分析
- 批准号:
12470234 - 财政年份:2000
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of micromechanisms of metastasis using NOD/SCID-hu and GFP-transfectant human colorectal carcinoma cells
使用 NOD/SCID-hu 和 GFP 转染人结直肠癌细胞分析转移的微观机制
- 批准号:
11557082 - 财政年份:1999
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Chemo-radio-gene therapy targeted by ionizing radiation using the EGR1 promoter
使用 EGR1 启动子通过电离辐射靶向化学放射基因治疗
- 批准号:
09671276 - 财政年份:1997
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Implantation of human colorectal carcinoma cells in the liver and lymph nodes studied by in vivo fluorescence videomicroscopy
通过体内荧光视频显微镜研究人结直肠癌细胞在肝脏和淋巴结中的植入
- 批准号:
07671354 - 财政年份:1995
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Clinical and Experimental Studies on Growth and Metastasis of Human Osteosarcomas
人骨肉瘤生长和转移的临床与实验研究
- 批准号:
07407042 - 财政年份:1995
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Basic and Clinical Studies on Development and Progression of the Human Osteosarcoma
人类骨肉瘤发生发展的基础与临床研究
- 批准号:
62440062 - 财政年份:1987
- 资助金额:
$ 18.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)