Implantation of human colorectal carcinoma cells in the liver and lymph nodes studied by in vivo fluorescence videomicroscopy

1.体内荧光显微镜观察：将人结直肠癌细胞接种到裸鼠脾脏中，在1区肝窦中捕获。超过90%的癌细胞在注射后24小时内死亡。结果：1.肝窦内癌细胞严重畸形，可能导致肝内癌细胞死亡（ClinExp Metastasis，14，1996）.大肠癌患者血液中游离癌细胞的基因诊断：采用RT-PCR技术检测大肠癌患者血液中游离癌细胞。这一结果是否能预测癌症术后复发，目前正在观察中。用鼠colon 26细胞的实验性肝转移：用鼠colon 26腺癌细胞建立实验性肝转移。Colon 26强表达CD 44，抗CD 44抗体可抑制肝集落的产生. CD 44阳性人结肠直肠癌细胞的建立：克隆人CD 44同种型CD 44 v3 -10并转染到CD 44阴性结肠直肠癌细胞系中。建立的CD 44 v3 -10阳性细胞系粘附于透明质酸盐，而亲本细胞系则不粘附。当皮下植入裸鼠中时，显示生长延迟（Surgical Forum，47，1996）。

1. In vivo fluorescence videomicroscopoy : Human colorectal carcinoma cells inoculated into the spleens of nude mice were trapped at the zone 1 hepatic sinusoids. More than 90% of carcinoma cells died in the liver within 24 hr of injection. Carcinoma cells showed severe deformity in the hepatic sinusoids, which may have caused cancer cell death in the liver (Clin Exp Metastasis, 14,1996).2. Genetic diagnosis of free cancer cells in the blood of patients with colorectal cancer : Free cancer cells were detected in the blood of patients with colorectal cancer by the RT-PCR technique. Whether or not this result can predict postoperative recurrence of cancer is now under observation.3. Experimental hepatic metastasis with murine colon26 cells : Experimental hepatic metastasis was established with the murine colon26 adenocarcinoma cells. Colon26 strongly expressed CD44 and an anti-CD44 antibody inhibited production of liver colonies.4. Establishment of CD44-positive human colorectal carcinoma cells : A human CD44 isoform, CD44v3-10, was cloned and transfected into a CD44-negative colorectal cancer cell line. The established CD44v3-10 -positive line adhered to hyaluronate while the parental line did not. The transformant showed delay in growth when implanted subcutaneously in nude mice (Surgical Forum, 47,1996).