Novel Hematopoietic Factors Produced by Human Undifferentiated Leukemia Cell Lines. and Their Biological Significance

MB-1细胞系来自慢性粒细胞白血病(CML)患者的母细胞瘤，可移植到裸鼠身上。在TPA处理的MB-1细胞培养上清液中检测到肥大细胞的生长刺激活性。由于刺激肥大细胞生长的细胞因子可能具有作用于造血干细胞的能力，因此有必要对MB-1来源的活性进行表征。虽然MB-1细胞的肥大细胞刺激生长活性的纯化和克隆仍在进行中，但我们的初步结果表明，这可能是一种对肥大细胞具有独特活性的新的细胞因子。采用体外培养系统建立了t(9；11)易位的单核细胞白血病细胞系IMS-M1。令人意外的是，大多数白血病样本都不能移植到SCID小鼠身上，这可能是因为缺乏合适的人类造血生长因子。其他研究课题包括利用基因转移技术进行髓样细胞的恶性转化及其特性、G-CSF诱导基因的分子克隆、可诱导分化的小鼠胚胎成纤维细胞系(C3H10T1/2)的造血支持能力分析、人胎肝来源的cDNA文库的构建等。

The MB-1 cell line, which was derived from the blastoma of a patient with chronic myelogenous leukemia (CML), is transplantable to nude mice. Mast cell growth-stimulating activity was detected in the culture supernatant of TPA-treated MB-1 cells. Since it is possible that cytokines which stimulate the growth of mast cells have the ability to act on hematopoietic stem cells, it is worth while characterizing the MB-1-derived activity. Although the purification and cDNA cloning of the mast cell growth-stimulating activity of MB-1 cells are still ongoing, our preliminary results suggested that this may be a novel cytokine with a unique activity on mast cells.We also attempted to establish human leukemia cell lines. The IMS-M1, a monoblastic leukemia cell line with t(9 ; 11) translocation, was established using an in vitro culture system. Unexpectedly, most leukemia samples ware not transplntable to SCID mice, probably due to the absence of appropriate human hematopoietic growth factors.The other research subjects include malignant transformation of myloid cells using a gene transfer technique and their characterization, molecular cloning of G-CSF-induced gene cDNA, analysis of hematopoiesis-supporting ability of a differentiation-inducible mouse embryo fibroblast cell line (C3H10T1/2), construction of cDNA library derived from human fetal liver, and so forth.