Expression and function of B cell specific MB-1 protein associated with IgM receptor

与 IgM 受体相关的 B 细胞特异性 MB-1 蛋白的表达和功能

• 批准号: 02454192
• 负责人: SAKAGUCHI Nobuo
• 金额: $ 3.65万
• 依托单位: Tottori University (1992)佐賀医科大学 (1990-1991)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454192/
• 关键词: IgM receptor; monoclonal Ab; signal transduction; pre-B cell; phosphorylation; lgMレセプター; IgMレセプタ-; モノクロ-ナル抗体; preーB細胞; pre B細胞

We have previously identified a novel B cell specific gene named mb-1 that is expressed selectively in the restricted stage of B cell differentiation. In this project, we identified the B cell specific mb-1 gene product and found the association of the MB-1 protein with IgM receptor on the surface of B cells. To further characterize the expression and function of this molecule, we prepared rat monoclonal Ab against MB-1 protein on the cell surface. MB-1 is expressed already at the earliest stage in the B cell differentiation when IgM receptor ganes are with germ line configuration. Using mAbs, we demonstrated that MB-1 molecule itself is capable of mediating positive signals into B lineage cells in proliferation, increased Ca^<2+> concentration, and in tyrosne phosphorylation of intracellular proteins. These results suggested interesting but unknown functions of the IgR-associated molecules in the differentiation of early B lineage cells. We are currently started to analyze further function of MB-1 molecule in the experimental systems with DNA transfection, and gene targeting method.

我们先前已经鉴定了一种新的B细胞特异性基因，命名为mb-1，其在B细胞分化的限制性阶段选择性表达。在本项目中，我们鉴定了B细胞特异性MB-1基因产物，并发现MB-1蛋白与B细胞表面的IgM受体相关。为了进一步研究该分子的表达和功能，我们在细胞表面制备了针对MB-1蛋白的大鼠单克隆抗体。MB-1在B细胞分化的最早阶段即已表达，此时IgM受体加内斯具有生殖系构型。使用单克隆抗体，我们证明MB-1分子本身能够在增殖、增加的Ca^2+浓度和细胞内蛋白质的酪氨酸磷酸化中介导阳性信号进入B谱系细胞。这些结果表明，有趣的，但未知的功能的免疫球蛋白受体相关的分子在早期B谱系细胞的分化。我们目前正在用DNA转染和基因打靶的方法在实验系统中进一步分析MB-1分子的功能。

项目成果

Takai,Y.: "Identification of IL-7-dependent bone marrow-derived Thy-1“B220"lymphoid cell clones thet rearrange and express both lg and Tcell receptor genes." J.Immunol.148. 1329-1337 (1992)

Takai, Y.：“IL-7 依赖性骨髓来源的 Thy-1“B220”淋巴细胞克隆的鉴定，它们重新排列并表达 lg 和 T 细胞受体基因。J.Immunol.148（1992）。

T.Matsuo: "Direct identification of the putative surface IgM receptorーassociated molecule encoded by murine B cellーspecific mbー1 gene." J.Immunol.(1991)

T.Matsuo：“直接鉴定由鼠 B 细胞特异性 mb-1 基因编码的推定表面 IgM 受体相关分子。J.Immunol。（1991）

Sakaguchi,N.: "Immunoglobulin receptor(lgR)-associated molecules." Advances in Immunology.

Sakaguchi,N.：“免疫球蛋白受体（lgR）相关分子。”

Takai,T.: "Identification of IL-7-dependent bone marrow-derived Thy-1^-B2200^-lyphoid cell clones thet rearrange and express both Ig and T cell receptor genes." J.Immunol.148. 1329-1337 (1992)

Takai,T.：“鉴定 IL-7 依赖性骨髓来源的 Thy-1^-B2200^-淋巴细胞克隆，它们重新排列并表达 Ig 和 T 细胞受体基因。”

Takai,Y.: "Identification of IL-7-dependent bone marrow-derived Thy-1^-B220^-lymphoid cell clones that rearrange and express both Ig and T cell receptor genes." J.Immunol.148. 1329-1337 (1992)

Takai,Y.：“鉴定 IL-7 依赖性骨髓来源的 Thy-1^-B220^-淋巴细胞克隆，这些细胞克隆重新排列并表达 Ig 和 T 细胞受体基因。”