Functions and behavior of a new type of ATPase(phosphatase) in halophytes

盐生植物中新型ATP酶（磷酸酶）的功能和行为

• 批准号: 03660087
• 负责人: HIRAYAMA Osamu
• 金额: $ 1.22万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660087/
• 关键词: halophyte; ATPase; phosphatase; membrane-bound enzyme; salt-resistant enzyme; ミクロソ-ム; 膜結合型酵素

A new type of ATPase or phosphatase was found in a halophyte, Solsola komaroli. It occurred in two forms of plasma membrane-bound and free, their activity ratio being 4/96. Both forms of the enzyme hydrolyzed phosphate ester of substrates in the following order ; p-nitrophenylphosphate > pyrophosphate > ADP * ATP > AMP. The activities of both enzymes were stimulated by mono- and di-valent cations, and they were strongly inhibited by molybdate and vanadate. From these properties, this enzyme should be accepted as a new type of acid phosphatase. Both forms of the enzyme were resistant to salinity, particularly the membrane-bound form was stimulated by high concentrations of salt. The present enzyme was also found in non-halophytes, soybean and cucumber, and it was distributed at an increasing concentration with an increase in salt tolerance of plants.

在盐生植物草莓中发现了一种新型的ATPase或磷酸酶。它以质膜结合和游离两种形式存在，其活性比为4/96。这两种形式的酶按以下顺序水解底物的磷酸酯：对硝基苯基磷酸盐；焦磷酸盐；ADP*ATP和GT；AMP。一价阳离子和二价阳离子对这两种酶的活性均有促进作用，而钼酸根和钒酸根离子对这两种酶的活性有较强的抑制作用。从这些性质来看，该酶应被认为是一种新型的酸性磷酸酶。这两种形式的酶都耐盐，特别是膜结合型受到高浓度盐的刺激。在非盐生植物、大豆和黄瓜中也发现了该酶，且随着植物耐盐性的增强，该酶呈浓度递增的分布。

项目成果

O.HIRAYAMA,M.NODA and T.TETSUO: "Characterization and distributation of acid phosphatase in a halophyte,saltwort" Phytochemistry,.

O.HIRAYAMA、M.NODA 和 T.TETSUO：“盐生植物盐藻中酸性磷酸酶的特征和分布”植物化学。

O.Hirayama and K.Nagaoka: "Separation of inside-out and right-side-out membrane vesicles from plasma-and tonoplast-membranes of soybean and saltwort" Plant cell Physiol.

O.Hirayama 和 K.Nagaoka：“从大豆和盐草的质膜和液泡膜中分离内向外和右外膜泡”植物细胞生理学。

平山 修,横田 一成,野田 政之,本多 哲郎: "塩生植物オカヒジキにおける膜結合性ホスファタ-ゼの存在とその特性" Agricultural and Biological chemistry.

Osamu Hirayama、Kazunari Yokota、Masayuki Noda、Tetsuro Honda：“盐生植物 Okahijiki 中膜结合磷酸酶的存在和特性”农业和生物化学。

O.HIRAYAMA and K.NAGAOKA: "Separation of inside-out and right-side-out membrane vesicles from plasma- and tonoplast-membranes of soybean and saltwort" Plant cell Physiol.

O.HIRAYAMA 和 K.NAGAOKA：“从大豆和盐草的质膜和液泡膜中分离内向外和右外膜泡”植物细胞生理学。

O.Hirayama,M.Noda and T.Tetsuo: "Characterization and distribution of acid phosphatase in a halophyte,saltwort" Phytochemistry,.

O.Hirayama、M.Noda 和 T.Tetsuo：“盐生植物、咸草中酸性磷酸酶的特征和分布”植物化学。