Studies on prevention of fouling by barnacles

藤壶污染防治研究

• 批准号: 03660200
• 负责人: KADO Ryusuke
• 金额: $ 0.58万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660200/
• 关键词: Prevention of fouling; Barnacles; Bioassay; Antifoulants; fatty acid from porcine liver; タテジマフジツボ; 再生産条件; キプリス幼生; 生物検定

1. Reproductive condition for Balanus amphitrite.It became possible to get hatched nauplii every day under the following rearing conditions : Salinity is about 27, water temperature between 21 to 25 C, and natural light condition. Exchange of seawater is needed once a week at least. It is essential to dry up of brood adults for several hours a day. More than eight hundred of hatched Artemia should be given for a barnacle in a day.2. Methods of bioassay using B. amphitrite cyprids.Hatched barnacle nauplii could be metamorphosed to cyprids by Chaetoceros calcitrans as food in 22 to 25 C. The cyprids for bioassay are those kept in 5 C for 2 to 10 days after the metamorphose. Assay vessel is used polystyrene or glass petri dishes. Inside the vessels of test lot are coated with solubilized test substance, and let solvent volatilize. Twenty cyprids and 5 ml seawater are introduced to the vessel. The seawater is that the salinity is above 20, filtered with glass filter (GF/C) and UV radiated. The vessels are incubated for 3 to 4 days under continuous dark and 25 C. Number of attached cyprid and juvenile are counted at the end of incubation.3. Screening of various substances as antifoulants(1) Prevention of fouling by applying antifoulant to the substratum.Among the various substances tested, fatty acid from porcine liver (FAPL) and calcium alginate were noticed to have high antifouling activity without killing the larvae. Since the latter inhibited settlement of cyprids more than 80 %, the antifouling activity in the sea is now testing in Mutsu Bay.(2) Prevention of fouling by dissolving antifoulant to the sea.Carbonic acid was tested instead of chlorine used as dissolving antifoulant before. By aerating carbonic acid gas into seawater, pH of the seawater during test period decreased below 6.6, as a result, settlement of cyprids was perfectly inhibited for at least four days.

1.藤壶的繁殖条件：盐度27左右，水温21 ~ 25 ℃，自然光照条件下，每天都能孵化出无节幼体。每周至少需要更换一次海水。这是必不可少的干燥育雏成人每天几个小时。一只藤壶一天需要800多只孵化出来的卤虫。使用B的生物测定方法。在22 ~ 25 ℃的温度下，角毛藻可将孵化的藤壶无节幼体转化为鲤形幼体。用于生物测定的Cyprids是在变质后在5 ℃下保持2至10天的那些。试验容器使用聚苯乙烯或玻璃培养皿。在供试批次的容器内涂上溶解的供试品，并使溶剂挥发。将20只塞浦路斯鱼和5 ml海水引入容器中。海水是盐度在20以上，用玻璃过滤器（GF/C）过滤，紫外线照射。将容器在连续黑暗和25 ℃下孵育3 - 4天.孵化结束后，统计附着的鲤和幼鱼数量.（1）通过将防污剂应用于底质来防止污染在所测试的各种物质中，注意到猪肝脂肪酸（FAPL）和海藻酸钙具有高的防污活性而不杀死幼虫。由于后者抑制了80%以上的塞浦路斯人的定居，海洋中的浮游生物活动现在正在陆津湾进行测试。(2)将防污剂溶解于海水中进行防污试验，试验用碳酸代替氯作为溶解性防污剂。通过向海水中充入碳酸气，使试验期间海水的pH值降至6.6以下，从而完全抑制了塞浦路斯的沉降至少4天。

项目成果

Rina Goto, Ryusuke Kado, Koji Muramoto and Hisao Kamiya: "Fatty acids as antifoulants in a marine sponge" Biofouling. 6. 61-68 (1992)

Rina Goto、Ryusuke Kado、Koji Muramoto 和 Hisao Kamiya：“脂肪酸作为海绵中的防污剂”生物污损。

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Antifouling activity of fatty acids found in marine sponge." Biofouling.

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“海绵中脂肪酸的防污活性。”

Rina GOTO,Michio MURATA,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "A barnacle settlement inhibitor from a marine sponge Phyllospongia papyracea" 日本農芸学会誌.

Rina GOTO、Michio MURATA、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“来自海洋海绵 Phyllospongia papyracea 的藤壶沉降抑制剂”日本农业科学学会杂志。

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Fatty acids as antifoularts in a marine sponge." Biofouling. 6. 61-68 (1992)

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“脂肪酸作为海洋海绵的防污剂。”

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Fatty acids as antifoulants in a marine sponge." Biofouling. 6. 61-68 (1992)

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“脂肪酸作为海绵的防污剂。”