Studies on prevention of fouling by barnacles

藤壶污染防治研究

• 批准号: 03660200
• 负责人: KADO Ryusuke
• 金额: $ 0.58万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660200/
• 关键词: Prevention of fouling; Barnacles; Bioassay; Antifoulants; fatty acid from porcine liver; タテジマフジツボ; 再生産条件; キプリス幼生; 生物検定

1. Reproductive condition for Balanus amphitrite.It became possible to get hatched nauplii every day under the following rearing conditions : Salinity is about 27, water temperature between 21 to 25 C, and natural light condition. Exchange of seawater is needed once a week at least. It is essential to dry up of brood adults for several hours a day. More than eight hundred of hatched Artemia should be given for a barnacle in a day.2. Methods of bioassay using B. amphitrite cyprids.Hatched barnacle nauplii could be metamorphosed to cyprids by Chaetoceros calcitrans as food in 22 to 25 C. The cyprids for bioassay are those kept in 5 C for 2 to 10 days after the metamorphose. Assay vessel is used polystyrene or glass petri dishes. Inside the vessels of test lot are coated with solubilized test substance, and let solvent volatilize. Twenty cyprids and 5 ml seawater are introduced to the vessel. The seawater is that the salinity is above 20, filtered with glass filter (GF/C) and UV radiated. The vessels are incubated for 3 to 4 days under continuous dark and 25 C. Number of attached cyprid and juvenile are counted at the end of incubation.3. Screening of various substances as antifoulants(1) Prevention of fouling by applying antifoulant to the substratum.Among the various substances tested, fatty acid from porcine liver (FAPL) and calcium alginate were noticed to have high antifouling activity without killing the larvae. Since the latter inhibited settlement of cyprids more than 80 %, the antifouling activity in the sea is now testing in Mutsu Bay.(2) Prevention of fouling by dissolving antifoulant to the sea.Carbonic acid was tested instead of chlorine used as dissolving antifoulant before. By aerating carbonic acid gas into seawater, pH of the seawater during test period decreased below 6.6, as a result, settlement of cyprids was perfectly inhibited for at least four days.

1。巴拉努斯（Balanus）两极的生殖状况。在以下饲养条件下，每天都有可能孵化nauplii：盐度约为27，水温在21至25 c之间，自然光条件。至少每周需要一次交换海水。每天要枯萎几个小时，至关重要。一天应该给出八百多个孵化的artemia。使用B. cyprids的生物测定方法。可以用chaetoceros calcitrans作为22至25 C的食物将搭配的藤壶nauplii变成Cyprids。生物测定的塞普里德群是在Metamorphose后5 C至10天的生物测定中的塞浦路斯。测定容器用于聚苯乙烯或玻璃培养皿。测试批的容器内涂有溶解的测试物质，并让溶剂挥发。将二十个塞浦路斯和5毫升海水引入船只。海水是盐度高于20，用玻璃过滤器（GF/C）过滤，并辐射紫外线。在连续的黑暗和25 C下将血管孵育3至4天。在孵化结束时计数附着的Cyprid和少年的数量3。筛选各种物质作为防腐剂（1）通过在底层上施用抗原剂来预防污垢。由于后者抑制了超过80％的Cyprid的沉降，因此现在在Mutsu湾进行了抗染色活性。（2）通过将抗抗溶剂溶解到海中，预防污染，而不是氯于溶液，而不是以前用作溶解抗抗菌剂的氯。结果，通过将碳酸气体曝气到海水中，测试期间海水的pH值降至6.6以下，因此，塞普利斯的沉降至少被完全抑制了四天。

项目成果

Rina Goto, Ryusuke Kado, Koji Muramoto and Hisao Kamiya: "Fatty acids as antifoulants in a marine sponge" Biofouling. 6. 61-68 (1992)

Rina Goto、Ryusuke Kado、Koji Muramoto 和 Hisao Kamiya：“脂肪酸作为海绵中的防污剂”生物污损。

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Antifouling activity of fatty acids found in marine sponge." Biofouling.

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“海绵中脂肪酸的防污活性。”

Rina GOTO,Michio MURATA,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "A barnacle settlement inhibitor from a marine sponge Phyllospongia papyracea" 日本農芸学会誌.

Rina GOTO、Michio MURATA、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“来自海洋海绵 Phyllospongia papyracea 的藤壶沉降抑制剂”日本农业科学学会杂志。

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Fatty acids as antifoulants in a marine sponge." Biofouling. 6. 61-68 (1992)

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“脂肪酸作为海绵的防污剂。”

Rina GOTO,Ryusuke KADO,Koji MURAMOTO and Hisao KAMIYA: "Fatty acids as antifoularts in a marine sponge." Biofouling. 6. 61-68 (1992)

Rina GOTO、Ryusuke KADO、Koji MURAMOTO 和 Hisao KAMIYA：“脂肪酸作为海洋海绵的防污剂。”