DESTRUCTION OF SYNOVIAL CELLS BY EB VIRUS-USING POLYMERASE CHAIN REACTION METHOD

本研究利用EBNA-1基因的引物，用聚合酶链式反应(PCR)方法检测了类风湿关节炎(RA)患者鼻窦组织中EB病毒(EBV)DNA，但用逆转录聚合酶链式反应(RT-PCR)检测不到EB病毒(EBV)-DNA的表达，并发现由EBNA-2基因反式激活的可溶性CD23(SCD23)在RA患者血清中浓度显著高于正常人。此外，我们通过原位杂交在1例RA患者III型滑膜细胞中发现了内部重复序列1 EBV-DNA信号，而在另外3例RA患者和1例骨关节炎患者的滑膜细胞中未发现EBV-DNA信号。这是首次在RA患者的鼻窦细胞中发现EBV的存在。

This project demonstrated that EB virus(EBV)-DNA was detected by polymerase chain reaction(PCR) method using EBNA-1 primers in sinovial tissue from the patients with rheumatoid arthritis(RA), but not detected mRNA by reverse transcriptase PCR(RT-PCR), and the concentration of solble CD23(sCD23) which was trans-activated by EBNA-2 gene, was significant higher in sera from patients with RA than normal individuals. Furthermore we found internal repeat-1 EBV-DNA signals by in situ hybridization in synovial cells which might be spindle type, from type III synovium of a patient with RA but not in another 3 RA and a osteoarthritis patients.This is a first report that has demonstrated the existence of EBV in sinovial cells from patient with RA.Further investigation will clarify the association between EBV and pathgenesis of RA.