DESTRUCTION OF SYNOVIAL CELLS BY EB VIRUS-USING POLYMERASE CHAIN REACTION METHOD

EB病毒聚合酶链反应法对滑膜细胞的破坏

• 批准号: 03670341
• 负责人: SAWADA Shigemasa
• 金额: $ 1.34万
• 依托单位: NIHON UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670341/
• 关键词: rheumatoid arthritis; EB virus; sCD23; sinovial cell; in situ hybridization; polymerase chain reaction; osteoarthritis; mRNA; 変形性関節症; EBウィルス; RT-PCR法; RA滑膜; EBNA-1 mRNA; プライマー; EpsteinーBarrウイルス; EBウイルスDNA; EBウイルスRNA

This project demonstrated that EB virus(EBV)-DNA was detected by polymerase chain reaction(PCR) method using EBNA-1 primers in sinovial tissue from the patients with rheumatoid arthritis(RA), but not detected mRNA by reverse transcriptase PCR(RT-PCR), and the concentration of solble CD23(sCD23) which was trans-activated by EBNA-2 gene, was significant higher in sera from patients with RA than normal individuals. Furthermore we found internal repeat-1 EBV-DNA signals by in situ hybridization in synovial cells which might be spindle type, from type III synovium of a patient with RA but not in another 3 RA and a osteoarthritis patients.This is a first report that has demonstrated the existence of EBV in sinovial cells from patient with RA.Further investigation will clarify the association between EBV and pathgenesis of RA.

本研究证实，在类风湿关节炎（RA）患者的鼻窦炎组织中，利用EBNA-1引物，聚合酶链反应（PCR）方法检测到EB病毒(EBV)-DNA，而逆转录酶PCR（RT-PCR）方法未检测到mRNA，且类风湿关节炎患者血清中EBNA-2基因反式激活的可溶性CD23（sCD23）浓度显著高于正常人。此外，我们通过原位杂交在一名RA患者的III型滑膜细胞中发现了内部repeat-1 EBV-DNA信号，这些细胞可能是纺锤型，但在另一名RA患者和一名骨关节炎患者中没有发现。这是第一个证明eb病毒存在于风湿性关节炎患者的窦细胞中的报告。进一步的研究将阐明EBV与RA发病机制之间的关系。

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