Quantitative analysis of acute ethanol induced changes in hepatic microcirculation

急性乙醇引起的肝脏微循环变化的定量分析

• 批准号: 03670378
• 负责人: HAMAMATSU Hisayoshi
• 金额: $ 1.28万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670378/
• 关键词: Liver; Microcirculation; Ethanol

Although the hepatic microcirculation is thought to be closely related to the liver function, there has been much controversy about the effect of ethanol on microcirculation. The present study was undertaken to quantitate changes in hepatic microcirculation after acute ethanol administration. To that effect, male rats were anesthetized with sodium pentobarbital(35 mg/kg bw) intraperitoneally. After laparotomy, a lobe of the liver was exposed on the slide glass at the window of metal plate for observation with an intravital inverted microscope assisted by SIT (silicon intensified target) camera. Erythrocytes were labeled with fluorescein isothiocyanate (FITC) according to the method of Zimmerhackl et al (1983), and injected from the catheter placed in the left carotid artery. 20% ethanol and 40% (3.0 g/kg bw and 6.0, respectively) were then injected through the stomach tube. The fluorescent microscopic image of the hepatic microcirculation was recorded on a videotape. The flow velocity … More of individual RBCs in sinusoids was measured with multipurpose computerized image analysis system for microcirculation (IM-Capiflow, Sweden) from playback of recorded images. Portal pressure, central vein pressure and mean arterial pressure were measured with the catheter cannulated in portal vein, central vein and the left carotid artery respectively. The flow velocity increased about 72% at 10-20 min after injection of 20"% ethanol and remained increased up to 60 min. But, the flow velocity after 40% ethanol decreased about 30 min after injection. Portal pressure increased about 18% and 24% after 20% ethanol and 40% respectively, but central vein pressure and mean arterial pressure had no significant change. In summary, acute oral administration of 20% ethanol increased portal pressure and the blood flow in hepatic microcirculation. On the other hand, that of 40% ethanol increased portal pressure but decreased the blood flow in hepatic microcirculation. These results suggested that microcirculatory disturbance was caused by changes in the liver induced by ethanol at high concentration. Less

肝脏微循环与肝功能密切相关，但乙醇对肝脏微循环的影响一直存在争议。本研究旨在定量急性乙醇给药后肝脏微循环的变化。为此，用戊巴比妥钠（35 mg/kg bw）腹腔内麻醉雄性大鼠。剖腹后，在活体倒置显微镜下，在金属板窗口的载玻片上暴露肝叶，并辅以SIT（硅增强靶）摄像机进行观察。根据Zimmerhackl等（1983）的方法，用异硫氰酸荧光素（FITC）标记红细胞，并从放置在左颈动脉中的导管注射。然后通过胃管注射20%乙醇和40%乙醇（分别为3.0 g/kg bw和6.0 g/kg bw）。将肝脏微循环的荧光显微图像记录在录像带上。流速 ...更多信息 用微循环多用途计算机图像分析系统（IM-Capiflow，Sweden）从记录的图像回放测量窦状隙中单个RBC的浓度。分别在门静脉、中心静脉和左颈动脉插管，测量门静脉压、中心静脉压和平均动脉压。注射20%乙醇后10-20 min流速增加约72%，并持续增加至60 min，而注射40%乙醇后30 min流速下降。20%和40%乙醇组门静脉压分别升高18%和24%，而中心静脉压和平均动脉压无明显变化。总之，急性口服20%乙醇增加门静脉压力和肝微循环血流量。而40%乙醇则使门静脉压力升高，肝微循环血流量减少。结果表明，高浓度乙醇引起的肝脏微循环障碍是由肝脏的变化引起的。少

项目成果

浜松 永昌,加藤 眞三 末松 誠他: "急性エタノール投与時の肝微小循環動態の解析ーFITC標識赤血球を用いた検討ー" 日本消化器病学会雑誌. 88. 2053- (1991)

Nagamasa Hamamatsu、Shinzo Kato、Makoto Suematsu 等人：“急性乙醇给药期间肝脏微循环动力学的分析 - 使用 FITC 标记的红细胞的研究 -”日本胃肠病学会杂志 88。2053-（1991）。

H.Hamamatsu: "Quantitative analysis of acute ethanol induced changes in hepatic microcirculation" Alcohol and Alcoholism 27. 77. (1992)

H.Hamamatsu：“急性乙醇引起的肝脏微循环变化的定量分析”酒精和酒精中毒 27. 77. (1992)

浜松 永昌,加藤 眞三 末松 誠 他: "急性エタノール投与時の肝微小循環動態の解析ーFITC標識赤血球を用いた検討ー" 日本消化器病学会雑誌. 88. 2053- (1991)

Eimasa Hamamatsu、Shinzo Kato、Makoto Suematsu 等人：“急性乙醇给药期间肝脏微循环动力学的分析 - 使用 FITC 标记的红细胞的研究 -”日本胃肠病学会杂志 88。2053-（1991）。

浜松 永昌,加藤 眞三,末松 誠 他: "急性エタノール投与時の肝微小循環動態の解析ー特にエタノール濃度との関係についてー" 日本消化器病学会雑誌. 89. 312- (1992)

Eimasa Hamamatsu、Shinzo Kato、Makoto Suematsu 等人：“急性乙醇给药期间肝脏微循环动力学分析 - 特别是与乙醇浓度的关系”日本胃肠病学会杂志 89. 312- (1992)。

H.Hamamatsu,S.Kato M.Suematsu,H.Ishii,et al: "Quantitative analysis of acute ethanol induced changes in hepatic microcirculation" Alcohol and Alcoholism. 27. 77- (1992)

H.Hamamatsu、S.Kato M.Suematsu、H.Ishii 等人：“急性乙醇引起的肝微循环变化的定量分析”酒精和酒精中毒。