GVHR IN COMBINED SMALL BOWEL AND LIVER TRANSPLANTATION

使用体重为200-300 g的纯种雄性刘易斯（Lew）和LewisXBrown Norway F1杂种（LBNF 1）大鼠。采用Monchik和Russel技术进行异位小体积移植。在研究的第一部分，进行了移植物淋巴细胞的示踪。在腹膜内注射20 mg溴脱氧尿苷（BrdU）后，将小肠解剖作为移植物。GVHR组（Lew大鼠为供体，LBNF 1大鼠为受体）行异位小肠移植。移植后第5天处死动物。取小肠粘膜标本和肠系膜淋巴结。切片用ABC法染色。采用抗BrdU抗体和抗淋巴细胞表面标志物（W3/13），研究第二部分，将动物分为两组，研究免疫细胞动力学。第一组为同种异体移植组作为对照。LBNF 1 ra ...更多信息 ts充当捐赠者和接受者。第二组为排斥组。LBNF 1大鼠作为供体，Lew作为受体。第三个是GVHR。Lew大鼠作为供体，LBNF 1大鼠作为受体。用流式细胞仪检测Peyer结和肠系膜淋巴结的BrdU标记指数和DNA合成时间，证实GVHR时自体肠系膜淋巴结中有BrdU阳性淋巴细胞从移植物中迁移。在GVHR中，淋巴结中的W3/13阳性细胞显著增加。这些同种异体T淋巴细胞被刺激并产生针对天然精氨酸的免疫应答4）。BrdU标记指数和DNA合成时间的测定表明，移植肠淋巴系统受到刺激，而自体肠淋巴系统受到强烈抑制，提示移植肠粘膜屏障功能减弱。这会导致细菌和抗原进入门静脉系统，有时会导致接受者出现脓毒症。小肠肝联合移植可降低移植物的抗原性。少

Purebred male Lewis (Lew) and LewisXBrown Norway F1 hybrid (LBNF1) rats weighting 200-300 g were used. The heterotopic small bowei transplantation was carried out using the technique of Monchik and Russel. For the first part of investigation, the tracing of lymphatic cells from grafts was performed. The small bowel was dissected as a graft after intra-peritoneal injection of 20 mg of bromodeoxyuridine (BrdU). The heterotopic small bowel transplantation was carried out in GVHR group (Lew rats served as doner, and LBNF1 rats as recipient). The animals were sacrificed on the fifth day after transplantation. The mucosal specimens and mesenteric lymph nodes from the native intestine were obtained. The sections were stained with ABC method. Anti-Br dU antibody and anti-surface markers of lymphocytes (W3/13) were used.For the second part of investigation, the animals were divided in two groups to investigate the immunological cell kinetics. The first was iso graft group as a control. LBNF1 ra … More ts served as doner and recipient. The second was rejection group. LBNF1 rats served as doner and Lew served as recipient. The third was of GVHR. Lew rats served as doner, and LBNF1 rats as recipient. The animals were sacrificed on the eighth day after administration of 20 mg of Br dU. Br dU labelling index and DNA synthesis time of both Peyer's patch and mesenteric lymph node were examined with flowcytometer.We documented that the specimens from the native mesenteric lymph nodes include some Br dU positive lymphocytes that immigrated from the graft in GVHR. There was a marked increase of W3/13 positive cells in lymph nodes in GVHR These allogenic T lymphocytes become stimulated and develop an immune response against the native intestine4). We also documented that the Br dU labeling index and DNA synthesis time demonstrated that the lymphatic system of the graft was stimulated, but those of the native intestine was strongly suppressed in GVHR.These findings suggest that the diffuse barrier function of the mucosal linigs of the native intestine had weakened in GVHR. This results in bacteria and antigens breaking into the portal system, and sometimes causing recipients to develop a septic condition. The combined (small bowel and liver) transplantation may be useful for small bowel transplantation to decrease antigenesty of small bowel graft. Less