Morphogenesis of fibrous long-spacing collagen and tumor metastasis

电镜下应用免疫组化方法，对胶原酶处理的新生小鼠皮肤、神经纤维瘤和培养成纤维细胞的纤维长间距胶原（FLS）中I、III、IV、V、VI型胶原、层粘连蛋白和纤维连接蛋白的分布进行了分析，以阐明纤维的形态发生和来源。在酶处理的小鼠真皮中，我们观察到VI型胶原和层粘连蛋白的存在。VI型胶原分布在直径约6 nm的细纤维中，在基底层和胶原纤维附近有不成形的物质，在FLS的纵向排列的纤维中。在层粘连蛋白染色中，我们观察到抗体与毛细血管基底层和基底层细胞的反应更为阳性。FLS为周期性为100nm的电子致密非晶材料，其暗带被密集而特异地染色。在神经鞘瘤中，我们观察到层粘连蛋白的存在，但没有其他的。层粘连蛋白分布在毛细血管的基底层、雪旺细胞的部分基底层和FLS的暗带。在培养成纤维细胞的基质中，我们观察到VI型胶原的存在。这一结果与布朗的研究相吻合。研究层粘连蛋白的分布，没有层粘连蛋白。这些结果表明，FLS的暗带可能是由胶原酶部分消化的基底层分解产物在含VI型胶原的纤维上沉淀形成的，周期间隔约为90nm。

We are analyzed the distribution of type I , III,IV,V,VI collagens, laminin and fibronectin in fibrous long-spacing collagen (FLS) of collagenase-treated newborn mouse skin, schwnnoma and cultured fibroblasts by immunohistochemistry at the electron microscopic level to clarify the morphogenesis and origin of the fibers.In the enzyme-treated mouse dermis, we observed the presence of type VI collagen and laminin. The distribution of type VI collagen were recognized in thin filaments with a diameter of 6 nm, unformed material in the vicinity of basal laminae and collagen fibers and the longitudinally aligned filaments of FLS.In staining of laminin, we observed the more positive reaction with the antibody on the basal laminae of capillaries and basal layr cells. The dark bands of FLS, which were electron-dense amorphous materials with a periodicity of 100nm, were stained densely and specifically.In the schwannoma, we observed the presence of laminin, but not the others. The distribution of laminin were recognized in basal laminae of capillaries, some basal laminae of schwan cell and dark bands of FLS.In the matrix of culltured fibroblasts, we observed the presence of type VI collagens. This results corresponded with the Brun's study. Studying of the distribution of laminin, there aren't laminin.These results indicate that the dark bands of FLS may be formed by precipitation of breakdown products from the demal basal laminae partially digested by collagenase at the type VI collagen-contained filaments with the periodic intervals of about 90nm.