Bromodeoxyuridine 標識法による膀胱前癌病変の観察

溴脱氧尿苷标记法观察膀胱癌前病变

• 批准号: 03670742
• 负责人: 八竹 直
• 金额: $ 1.22万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670742/
• 关键词: 膀胱癌; 前癌病変; Bromodeoxyuridine(BrdU)

膀胱の前癌病変の把握に、bromodeoxyuridine(BrdU)標識法による、S期細胞の比率や分布様式の観察が有用であるかを検討した。雄ラット5匹に0.05%BBNを10週間摂取させ、50mg/KgのBrdUを腹腔内に投与した2時間後に膀胱を摘出、PAP法によりBrdU陽性細胞を同定し観察した。組織学的に、単純性過形成(S)を73、乳頭状あるいは結節状過形成(PN)を57、乳頭腫(Pa)を14病巣観察したが、陽性細胞(S期細胞)の比率の点では、組織のgrade upに比例した有意の増加は認められなかった。陽性細胞の分布様式の検討では、基底膜と離れた細胞(非基底細胞)における陽性所見の有無、及び、基底膜上における陽性細胞の連続性の有無を観察した。その結果、非基底細胞における陽性所見が得られた割合は、Sで11%(8/73)、PNで60%(33/57)、Paで100%(14/14)であった。また、基底膜上で陽性細胞が連続して観察できた病巣の割合は、Sで6%(4/73)、PNで25%(14/57)、Paで50%(7/14)であった。これらはいずれも有意の増加を示していた。以上の結果より、S→PN→Paという発癌の初期の過程で、非基底部分のS期細胞や、基膜評上で連続するS期細胞の出現することが特長的な所見と考えられ、このような観察が癌化の危険を早期に知る手懸かりになると思われた。

Bladder precancerous lesions were identified by bromodeoxyuridine (BrdU) labeling method and S-phase cell ratio distribution. In male, 5 0.05%BBN samples were harvested within 10 minutes, 50mg/Kg BrdU was injected intraperitoneally, and bladder was removed 2 hours later, and BrdU cells were examined by PAP method. Histological studies showed that the formation of sex (S) 73, the formation of breast cancer (PN) 57, the detection of breast cancer (Pa) 14, the percentage of sex cells (S phase), the proportion of tissue grade up (S), and the percentage of breast cancer cells (S cells) were significantly increased. The distribution of sexual cells is similar to that of the cells of the basement membrane (non-basal cells), and the connection of the cells on the basement membrane is observed. The results of the experiment and the results of non-basal cell disease were as follows: cut-off rate, 11% (8amp 73), 60% (33pm, 57), and 100% (14max, 14) respectively. The sexual cells on the basement membrane were linked and detected. The disease was cut off, Shear 6% (4 amp 73), PN 25% (14 max 57), Paver 50% (7 pm 14). Please tell me that you are interested in adding a few words to show your intention. The results of the above results showed that the early stage of cancer in the early stage of cancer, the S phase cell in the non-basal part, the S phase cell in the basement membrane link in the basement membrane, and the early stage of cancer risk were detected by the above results, and the early stage of cancer risk was detected.