Study of epidermal growth factor receptor in the testis.

FSH和睾酮被认为在精子发生的控制中起着重要作用。表皮生长因子(EGF)是由小鼠颌下腺分泌的一种生长因子，最近被报道对精子发生起到控制作用。如果这种现象是普遍存在的，那么这种因子的受体应该存在于睾丸中。此外，这些受体的鉴定将建立精子发生和EGF之间的直接关系。用生化和免疫组织化学方法分析动物和人类睾丸组织中的EGF受体。取C3H小鼠睾丸组织，以β&lt；125&gt；i-EGF为配体，对小鼠睾丸组织匀浆中的膜部分进行生化饱和分析。虽然改变了孵育时间和配体浓度等实验条件，但并未观察到β-表皮生长因子与睾丸组织的特异性结合。另一方面，ABC技术…更多的QUE，利用小鼠抗人EGF受体和单抗，证明了EGF受体存在于人和小鼠的支持细胞中。在我们发现的第一份年度报告中，我们提出EGF通过支持细胞在精子发生中发挥作用。然而，随着研究过程中病例的进一步积累，出现了许多EGF受体无法鉴定的病例；这些受体的存在无法得到证实。对特发性生精障碍和精索静脉曲张患者的睾丸组织标本进行分析，没有发现任何证据表明EGF受体的存在。分析精浆中精子发生与EGF的关系。对部分射精获得的精液的评估表明，精浆中的EGF来自前列腺癌。由于精浆中的EGF浓度与精子密度无关，因此我们认为精浆中的EGF对精子发生的作用不大。较少

FSH and testosterone are thought to play important roles in the control of spermatogenesis. Epidermal growth factor (EGF), which is secreted by the submandibular gland of the mouse, has recently been reported to contribute to the control of spermatogenesis. If this phenomenon is universal, then receptors for this factor should be present in the testis. Moreover, the identification of such receptors would establish a direct relationship between spermatogenesis and EGF.An analysis of EGF receptors in animal and human testicular tissues by biochemical and immunohistochemical methods was performed. Testicular tissues were obtained from C3H mice.Biochemical saturation analysis of the membranous fraction from a homogenate of mouse testicular tissues was performed with ^<125>I-EGF as a ligand. Although experimental conditions such as incubation time and ligand concentration were altered, no specific binding was noted between ^<125>I-EGF and testicular tissues.On the other hand, the ABC techni … More que, employing mouse anti-human EGF receptors and monoclonal antibodies, demonstrated EGF receptors in human and mouse Sertoli cells. In our first annual report of our findings, we suggested that EGF played a role in spermatogenesis via the Sertoli cells. However, as cases were further accumulated in the course of our study, there appeared many cases in which EGF receptors could not be identified; the existence of such receptors could not be confirmed. Analysis of specimens of testicular tissues obtained from patients with idiopathic impairments of spermatogenesis and varices of the spermatic cord failed to reveal any evidence of the existence of EGF receptors. Seminal plasma was analyzed with respect to the relationship between spermatogenesis and EGF. An evaluation of spermatic fluid obtained from fractional ejaculation showed that EGF in seminal plasma is of prostatic origin. Since the EGF concentration in seminal plasma did not correlate with sperm concentration, we concluded that seminal plasma EGF contributes little to spermatogenesis. Less